Managing Sequence Detection System Data - Applied Biosystems 7900HT User Manual

Managing Sequence Detection System Data

System
Operation and
Dataflow
Standard Modes
of Operation
GR2179
7900HT raven
instrument front
7900HT FAST Real-Time PCR System
Applied Biosystems 7900HT
Fast Real-Time PCR System
Instrument Firmware
3
Thermal Cycling and
Sequence Detection
4
Data Collection
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
Data management strategy is a crucial element of successfully integrating the
Applied Biosystems 7900HT Fast Real-Time PCR System into a laboratory
workflow. During a single 24-hour period of real-time operation, the 7900HT
instrument can produce more than 200 MB of data. To successfully manage the
information produced by the 7900HT instrument, you should have a basic
understanding of how data is collected, stored, and processed throughout the
operation of the instrument.
The Applied Biosystems 7900HT Fast Real-Time PCR System has three standard
modes of operation depending on the accessories purchased with the base instrument
• Stand-Alone Operation
• Automated Operation
• Database Operation
Stand-Alone Operation
This mode uses the most basic configuration of the Applied Biosystems 7900HT Fast
Real-Time PCR System
additional components. In this configuration, a technician runs plates individually using
the SDS software which stores all data on the computer hard drives.
Computer
SDS Software
Serial
Cable
Figure 1-11 Stand-Alone Operation of the 7900HT Instrument

Managing Sequence Detection System Data

(see below)
(see page 1-18)
(see page 1-18)
(Figure 1-11) where the 7900HT instrument is used without any
1
Plate Document
Created
2
Plate Document Used
to Run a Plate
5
Raw Data Saved to
the Plate Document
6
Plate Document
Opened and Analyzed
September 1, 2004 11:38 am, CH_Overview.fm
Hard drive(s)
*.sds *.sdt
file
DRAFT
file
1-17