Applied Biosystems 7900HT User Manual page 221

Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
• T display and slider – The SDS software displays the T
m
(see below).
T
m
There are two definitions for the T
– The chemical definition is the temperature at which 50% of the DNA is in a
double-stranded configuration.
– The mathematical definition is the maximum value for the first derivative
curve within a specific peak.
• Plot drop-down list – Chooses the data displayed within the plot based on the
derivative calculation. The list offers the following selections:
– Raw – When selected, this option plots the normalized reporter fluorescence
data (R ) on a graph of fluorescence vs. temperature (left-most plot in
n
Figure 6-16).
– Derivative – When selected, this option plots derivative data (R
of the derivative vs. temperature (right-most plot in
derivative data is the negative of the rate of change in fluorescence as a
function of temperature.
Figure 6-16 shows the plots accessible from the Plot drop-down list.
Raw Plot
Figure 6-16 Plots of the Dissociation Curve Tab
• Detector drop-down list – Chooses the data displayed within the plot based on
detector name.
Determining T
value:
m
DRAFT
September 1, 2004 11:39 am, CH_Real-Time.fm
Values for the Analyzed Run
m
below the green slider
m
´) on a graph
n
Figure 6-16). The
Derivative Plot
6-43