Applied Biosystems 7900HT User Manual page 82

Chapter 2 Getting Started
Tabs
2-30 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:38 am, CH_GetStart.fm
Plate documents can have up to six tabs (depending on their function) as described
below:
Table 2-5 Tabs of the SDS Software
Tab Function
Setup Displays well information, and allows you
to configure the plate grid with setup
information.
Instrument Used to program the plate document
method, run the plate document, or send it
to the Plate Queue.
Raw Data Displays the raw fluorescence collected
Plot from the sequence detection run.
The Raw Data tab is visible only in plate
documents containing run data.
To view the tab, open a plate document
containing run data, click
the Raw Data Plot tab.
Calibration Displays the Background and Pure Spectra
Data calibration data used for the signal
normalization and multicomponenting
analysis of the run data.
The Calibration Data tab is visible only in
plate documents containing run data.
To view the tab, open a plate document
containing run data, click
the Calibration Data tab.
Results Displays analyzed run data.
The Analysis tab is visible only in plate
documents containing analyzed run data.
Dissociation Displays analyzed dissociation curve data
Curve from a programmed ramp.
The tab is visible only in plate documents
containing analyzed data from a real-time
run with a programmed ramp.
DRAFT
Views/Plots
Well Inspector
• Method Editor
– Thermal Profile tab
– Auto Increment tab
– Ramp Rate tab
– Data Collection tab
• Real-Time tab
• Plate-Read tab
Raw Data Plot
, then select
Calibration Data
• Background Plot
• Pure Dyes Plot
, then select
Allelic Discrimination Data
• Allelic Discrimination Plot
Absolute Quantification
• Amplification Plot
• Standard (Curve) Plot
Relative Quantification
• Amplification Plot
Dissociation Plot