Analysis Checklist; Overview - Applied Biosystems 7900HT User Manual

Chapter 6 Analyzing Real-Time Data

Analysis Checklist

Workflow

Overview

6-22 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, CH_Real-Time.fm
1. Create an SDS 7900HT Multiple Plate Document (see
2. Add relative quantification plates for the analysis (see
3. Configure the analysis settings for the run (see
4. Analyze the run data (see
5. For each detector that you configured to determine the baseline and threshold
values (see page
– Automatically – Verify that the baseline and threshold values for the detector
were set correctly by the software.
– Manually – Set the baseline and threshold values for the detector.
6. For each detector that you configured for manual outlier removal, visualize outliers
and eliminate any outlying amplification from the run data (see
7. View the results of the relative quantification run (see
8. Choose from the following post-analysis settings (see
– Reanalyze the run data.
– Adjust the display settings for the results table, plate grid, and plate
document plots.
– Print elements of the plate document.
– Export the plate document results table or plots.
DRAFT
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