Applied Biosystems 7900HT User Manual page 85

Preparing a Run
In This Chapter
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
Notes for Database Users . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Before You Begin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4
Workflow Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
Quick Review: Powering On the 7900HT Instrument. . . . . . . . . . . . . . . . . . . . . . . 3-8
Step 1 - Creating a Plate Document . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-9
Step 2 - Applying Detectors and Markers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11
Step 3 - Configuring the Plate Document with Tasks . . . . . . . . . . . . . . . . . . . . . 3-16
Step 4 - Setting the Passive Reference and Omitting Wells . . . . . . . . . . . . . . . . . 3-18
Step 5 - Programming the Plate Document Method . . . . . . . . . . . . . . . . . . . . . . . 3-19
Step 6 - Saving the Plate Document as a Template. . . . . . . . . . . . . . . . . . . . . . . . 3-24
Step 7 - Creating a Plate Document from the Template . . . . . . . . . . . . . . . . . . . . 3-26
Step 8 - Applying Sample and Plate Information . . . . . . . . . . . . . . . . . . . . . . . . . 3-28
Step 9 - Running the Plate on the 7900HT Instrument. . . . . . . . . . . . . . . . . . . . . 3-29
DRAFT
September 1, 2004 11:39 am, CH_Run.fm
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