Specifying The Analysis Settings - Applied Biosystems 7900HT User Manual

Specifying the Analysis Settings

Specifying the
Settings for the
Analysis
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
1. Click (or select Analysis > Analysis Settings).
2. In the Detector drop-down list, select:
• detector name – To set the Analysis Settings for a specific detector.
• All Detectors – To set the Analysis Settings for a all detectors
simultaneously.
3. In the Analysis Settings dialog box, configure the method (automatic or manual)
that the software will use to determine the baseline and threshold values for the
selected detectors:
To determine the baseline and threshold values:
Automatically
Select the Automatic Ct option button. (See
Automatic Ct algorithm.)
If you choose to use the Automatic C
threshold were called correctly for each detector configured for AutoCalling
(following the procedure on
Manually
Do the following:
a. Select the Manual Ct option button.
b. In the Threshold field, enter a threshold value to apply to the selected
detector(s) or leave the field empty and set the threshold value manually as
explained on page
c. Select one of the following:
• Automatic Baseline, to have the software calculate a baseline for the
selected detector(s).
• Manual Baseline, then enter Start and End cycle values to apply to the
selected detector(s).
• Manual Baseline, and leave the Start and End cycles fields empty. (You
will need to set the values manually as explained on
4. If necessary, repeat
The threshold and baseline values set in
IMPORTANT!
and must be set for each individual detector used on in the plate document.
5. If evaluating groups of two or more replicates per sample, select or deselect the
Automatic Outlier Removal option.
6. If desired, select the Omit Samples Whose ∆C
value in the field. See
page 6-27 for more information.
7. In the Calibrator Sample drop-down list, select the sample that you want to use
as the calibrator for your study.
algorithm, verify that the baseline and
T
page 6-46).
6-46.
steps 2 through 3 for any additional detectors.
"Replicate and Outlier Removal for Study Settings" on
DRAFT
September 1, 2004 11:39 am, CH_Real-Time.fm
Analyzing the Study
page 6-18 for information on the
page 6-46.)
step 3 are detector specific,
<= check box, then enter a
T
6-29