Applied Biosystems 7900HT User Manual page 129

Loading the Fill
Reservoirs with
Sample-Specific
PCR Reaction
Mix
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
1. When the packaging has reached room temperature and you are ready to load
PCR reaction mix, carefully remove a Low Density Array from its packaging.
2. Place the Low Density Array on a lab bench, with the foil side down.
3. Load 100 µL of the desired sample-specific PCR reaction mix into a 100-µL
micropipette.
4. Hold the micropipette in an angled position and place the tip in the fill port.
There is a fill port on the left arm of each fill reservoir; it is the larger of
Note:
the two holes.
Do not allow the tip to contact and possibly damage the coated
IMPORTANT!
foil beneath the fill port.
5. Dispense the sample-specific PCR reaction mix so that it sweeps in and around
the fill reservoir toward the vent port.
Pipette the entire 100 µL into the fill reservoir.
IMPORTANT!
Do not allow the tip to contact and possibly damage the coated
IMPORTANT!
foil beneath the fill port.
IMPORTANT! Be careful when pushing the micropipette plunger to its second
stop position (to expel the sample-specific PCR reaction mix from the tip). If a
large amount of air is released, it can push the reaction mix out of the fill
reservoir via the vent port.
GR2159
6. To transfer the sample-specific PCR reaction mix from the fill reservoirs into
the reaction wells, the Low Density Array must be centrifuged.
Continue with "Centrifuging the TaqMan Low Density Arrays" on page
Preparing TaqMan Low Density Arrays for Use
Fill port
Vent port
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DRAFT
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