Running The Prepared Pure Dye Plate Or Card - Applied Biosystems 7900HT User Manual

Chapter 7 Maintaining the Instrument

Running the Prepared Pure Dye Plate or Card

Running the
Pure Dye Plate or
Card
7-24 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, CH_Maintenance.fm
1. Load the pure dye plate or microfluidic pure dye card into the 7900HT
instrument:
a. In the plate document in the SDS software, select the Instrument tab.
b. In the Real-Time tab in the Instrument tab, click
The instrument tray rotates to the OUT position.
c. Place the pure dye plate or card into the instrument tray as shown below.
The A1 position is located in the top-left side of the instrument tray.
Note:
2. Click .
The 7900HT instrument begins the pure dye run. The method for a pure dye run
is coded into the software and consists of a single 2-min hold at 60 °C.
Before starting the run, the instrument may pause (up to 15 min) to heat
Note:
the heated cover to the appropriate temperature.
3. When the pure dye run is complete and the Run Complete dialog box appears:
a. Click
b. Click
instrument tray.
c. Extract the pure dye calibration information as explained on
DRAFT
Well A1
Position the plate so that the bar code
faces towards the front of the instrument
Well A1
Position the microfluidic pure dye card so that the
bar code faces towards the front of the instrument
to close the dialog box.
, and remove the pure dye plate or card from the
.
page 7-25.