Applied Biosystems 7900HT User Manual page 142

Chapter 4 Operating the Instrument
Stopping the
Run Using the
SDS Software
4-28 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, CH_Basic.fm
Read the following directions carefully before stopping a
IMPORTANT!
run-in-progress.
Stopping End-Point Runs (Allelic Discrimination)
In the Plate-Read tab of the plate document, click
Stopping Real-Time Runs (Absolute or Relative Quantification)
Has the Cover reading in the Real-Time tab reached 104 °C?
• No (the heated cover temperature is below 104 °C)
In the Real-Time tab of the plate document, click
• Yes (the heated cover temperature has reached 104 °C)
The instrument is running the plate and has begun thermal cycling. Determine a
course of action from the options in the following table:
Reason for Stopping the Run
Forgot to make a change to the plate
document such as adding a detector,
detector task, or sample name
(not including mistakes to the
temperature profile)
• Forgot to add a reagent to the plate
(such as enzyme or master mix)
• Programmed the plate document
with the wrong thermal profile
DRAFT
.
.
Then...
do not stop the run.
Allow the instrument complete the run,
then edit the plate document before
analyzing the data. The software does not
use detector or sample information until
you analyze the plate document.
1. In the Real-Time tab of the plate
document, click
2. Determine how far into the run the
instrument has progressed.
3. Based on the state of the run, determine
whether the plate can be re-run.
4. If necessary, eject the plate and add the
missing reaction component.
5. If desired, re-run the plate by re-creating
the plate document.
.