Preparing Taqman Low Density Arrays For Use; About The Low Density Arrays - Applied Biosystems 7900HT User Manual

Chapter 4 Operating the Instrument

Preparing TaqMan Low Density Arrays for Use

About the Low Density Arrays

How It Works
Low Density
Array
Components
4-10 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, CH_Basic.fm
The TaqMan Low Density Arrays are research tools for profiling gene expression
using the Comparative C
evaluates from one to eight cDNA samples or controls generated from human total
RNA in a two-step RT-PCR experiment.
The Low Density Array functions as an array of reaction vessels for the
PCR/sequence detection step. Typically, the wells of the Low Density Array contain
Applied Biosystems fluorogenic 5´ nuclease assays (TaqMan
below) that detect the real-time amplification of user-specified targets. Relative
levels of gene expression are determined from the fluorescence data generated during
PCR using the Applied Biosystems 7900HT Fast Real-Time PCR System Relative
Quantification software.
In this document, the term TaqMan reagents refers to 5´ exonuclease assay
Note:
reagents that feature Applied Biosystems TaqMan
Applied Biosystems PDARs, TaqMan
Assays, Custom TaqMan
custom TaqMan reagents.)
The Low Density Array
a series of 384 interconnected wells divided into eight sets of assays. Each set
provides a user-specified number of replicates. Each well contains dried Applied
Biosystems TaqMan primers and probes for one mRNA target.
GR2156a
Figure 4-5 The TaqMan Low Density Array
DRAFT
Method of relative quantification. The Low Density Array
T
® SNP Genotyping and Gene Expression
® SNP Genotyping and Gene Expression Assays, and any
(Figure 4-5) acts as a vessel for real-time PCR. It consists of
® reagents; see the note
® primers and probes (includes
Fill reservoir (1 of 8)
A reservoir for the cDNA sample or
control before it is centrifugally
loaded into the wells
Fill consumable
The segment of the Low Density
Array that contains the eight fill
reservoirs. (After the Low Density
Array is centrifuged and sealed, the
fill consumable is trimmed off.)
Barcode Provides coded access to
Low Density Array configuration
databases