Applied Biosystems 7900HT User Manual page 105

Programming
Methods for
Absolute or
Relative
Quantification
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
The following procedure describes how to configure only the basic features of
Note:
the method: thermal cycler conditions, sample volume, and data collection options.
To further customize the method for the plate document, click
tab and refer to the online help for instructions on configuring the auto increment and
ramp rate values.
1. In the SDS software, select the Instrument tab of the plate document.
2. If necessary, select or de-select the 9600 Emulation check box.
When the 9600 Emulation check box is checked, the SDS software
Note:
reduces the ramp rate of the 7900HT instrument to match that of the
ABI P ® 7700 Sequence Detection System instrument.
RISM
3. Modify the default thermal profile for the method as needed:
To...
adjust step
parameters
(time/temp)
add a hold, cycle
set, or step
remove a step
Step 5 – Programming the Plate Document Method
Then...
select a field value, enter a new value, and click anywhere
outside of the field.
1. Click the step to the left of the location you want to place the
new stage.
2. Click or
The software inserts the stage into the thermal profile.
Note:
To add a step to a stage, select the step to the left of the
location you want to place the step and click
1. Click the step you want to remove.
The software highlights the selected step.
2. Click to remove the step from the profile.
September 1, 2004 11:39 am, CH_Run.fm
in the Instrument
Temperature field
(4 to 99.9 °C)
Time field
(0:01 to 98:59 min)
.
Selected step
New step
appears here
DRAFT
.
3-21