Applied Biosystems 7900HT Manuals

Manuals and User Guides for Applied Biosystems 7900HT. We have 2 Applied Biosystems 7900HT manuals available for free PDF download: User Manual, Getting Started Manual

Applied Biosystems 7900HT User Manual (384 pages)

Fast Real-Time PCR System and SDS Enterprise Database

Brand: Applied Biosystems | Category: Laboratory Equipment | Size: 8.7 MB

Table of Contents

3
- How to Use this Guide
  11
- Preface
  11
- How to Obtain more Information
  12
- How to Obtain Services and Support
  12
Safety and Emc Compliance Information

13
- Safety and EMC Compliance Information
  14
- Safety Conventions Used in this Document
  14
- Symbols on Instruments
  15
- Safety Labels on Instruments
  16
- General Instrument Safety
  17
- Chemical Safety
  18
- Chemical Waste Safety
  19
- Electrical Safety
  20
- Biological Hazard Safety
  21
- Laser Safety
  21
  - Safety
    21
- Physical Hazard Safety
  21
- Bar Code Scanner Laser Safety
  22
- Safety and Electromagnetic Compatibility (EMC) Standards
  23
- Workstation Safety
  23
1 Product Overview 1

25
- Chapter 1 Product Overview
  
  26
2 Getting Started 2

53
- Chapter 2 Getting Started
  
  54
3 Preparing a Run 3

85
- Chapter 3 Preparing a Run
  
  86
4 Operating the Instrument 4

115
- Chapter 4 Operating the Instrument
  
  116
5 Analyzing End-Point Data 5

159
- Chapter 5 Analyzing End-Point Data
  
  160
  - End-Point Runs on the 7900HT Instrument
    160
  - Notes for Database Users
    161
Section 5.1 Allelic Discrimination

163
- Overview
  164
- Before You Begin
  167
- Analysis Checklist
  168
- Opening the Run Data
  169
- Analyzing an Allelic Discrimination Run
  170
- Calling and Scrutinizing Allelic Discrimination Data
  171
- After the Analysis
  176
- Chapter 6 Analyzing Real-Time Data
  
  180
  - Notes for Database Users
    180
  - Real-Time Runs on the 7900HT Instrument
    182
Section 6.1 Absolute Quantification

183
- Overview
  184
- Before You Begin
  186
- Analysis Checklist
  187
- Opening the Run Data
  188
- Analyzing the Data
  189
- Viewing Results
  191
- After the Analysis
  192
- Section 6.2 Relative Quantification
  193
- Overview
  194
  - Algorithmic Manipulation of Raw Data
    195
  - Automatic Outlier Removal
    197
- Essential Experimental Components
  198
- Getting Started
  199
- Analysis Checklist
  200
  - Overview
    200
- Creating the Study
  201
- Options for Analyzing Relative Quantification Data
  201
- Analyzing the Study
  204
  - About the Analysis Settings
    204
  - Specifying the Analysis Settings
    207
  - Analyzing the Study Data
    208
  - Analyzing the Data
    208
- Viewing Results
  209
- After the Analysis
  213
Section 6.3 Dissociation Curve Analysis

215
- Overview
  216
- Before You Begin
  217
- Analysis Checklist
  218
  - Overview
    218
- Analyzing the Run Data
  219
- Opening the Run Data
  219
- Determining T Values for the Analyzed Run
  220
  - Values for the Analyzed Run
    220
- After the Analysis
  222
- Section 6.4 Procedure Reference
  223
- Setting the Baseline and Threshold Values
  224
- Eliminating Outlying Amplification
  227
- After the Analysis
  230
  - September 1, 2004 11:39 Am, Ch_Real-Time.fm
    230
7 Maintaining the Instrument 7

233
- Chapter 7 Maintaining the Instrument
  
  234
8 Troubleshooting 8

289
- Chapter 8 Troubleshooting
  
  290
Software Reference a

309
- Appendix A Software Reference
  
  310
  - Importing Plate Document Setup Table Files
    310
  - Setup Table Files
    312
    
    File Version
    313
    
    Plate Characteristics
    313
    
    Plate Size
    313
    
    Plate ID
    313
    
    Detector Definitions
    314
    
    Number of Detectors
    314
    
    Detectors List Header
    314
    
    Detectors List
    315
    
    Marker Definitions
    316
    
    Number of Markers (Allelic Discrimination Only
    316
    
    Markers List Header (Allelic Discrimination Only
    316
    
    Markers List (Allelic Discrimination Only
    317
    
    Well-Detector Information
    318
    
    10. Well-Detector List Header
    318
    
    11. Well-Detector Definition List
    318
    
    Well-Marker Information
    320
    
    Well-Marker List Header (Allelic Discrimination Only
    320
    
    Well-Marker Definition List (Allelic Discrimination Only
    320
    
    In this Appendix
    320
    
    Setup Table Files
    320
    
    Example Setup Table Files
    322
  - Exporting Graphics
    324
  - Exporting Plate Document Data
    324
  - Operating the SDS Software from a Command Line
    326
  - Using the Search Tool
    328
  - Connecting SDS Software to the Database
    330
- Appendix B Designing Taqman Reagent-Based Assays
  
  334
Kits, Reagents, and Consumables C

339

Applied Biosystems 7900HT Getting Started Manual (48 pages)

Micro Fluidic Card

Brand: Applied Biosystems | Category: Controller | Size: 0.76 MB

Table of Contents
3
Safety

5
- Safety Alert Words
  5
- Chemical Hazard Warning
  5
- Chemical Safety Guidelines
  5
- About Msdss
  6
- Obtaining Msdss
  6
- Site Preparation and Safety Guide
  7
- Waste Disposal
  7
How to Obtain Services and Support

7
Introduction

8
Materials and Equipment

11
Notes about Micro Fluidic Card Experiments

16
Performing Reverse Transcription

17
- Summary
  17
- Where You Are in the Procedure
  17
- Guidelines for Preparing RNA
  18
- Starting Amount of Total RNA
  18
- Storing Cdna
  18
Preparing and Running the Micro Fluidic Cards (PCR)

19
Performing Real-Time Data Analysis

33
- Summary
  33
- Where You Are in the Procedure
  33
- Absolute Vs. Relative Quantification
  34
Additional Tasks Related to Micro Fluidic Card Use

35
References

36
Appendix A Micro Fluidic Card Information CD

37
- About the Micro Fluidic Card Information CD
  37
- Assay Information File
  37
- Card Map Files
  40
- SDS Setup File
  41
Index

43

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More Applied Biosystems Manuals

Applied Biosystems 7900HT Manuals

Applied Biosystems 7900HT User Manual (384 pages)

Table of Contents

Table of Contents

Safety and Emc Compliance Information

1 Product Overview 1

Chapter 1 Product Overview

2 Getting Started 2

Chapter 2 Getting Started

3 Preparing a Run 3

Chapter 3 Preparing a Run

4 Operating the Instrument 4

Chapter 4 Operating the Instrument

5 Analyzing End-Point Data 5

Chapter 5 Analyzing End-Point Data

Section 5.1 Allelic Discrimination

Chapter 6 Analyzing Real-Time Data

Section 6.1 Absolute Quantification

Section 6.3 Dissociation Curve Analysis

7 Maintaining the Instrument 7

Chapter 7 Maintaining the Instrument

8 Troubleshooting 8

Chapter 8 Troubleshooting

Software Reference a

Appendix A Software Reference

Appendix B Designing Taqman Reagent-Based Assays

Kits, Reagents, and Consumables C

Appendix C Kits, Reagents, and Consumables

Appendix D Theory of Operation

Appendix E Limited Warranty Statement

Applied Biosystems 7900HT Getting Started Manual (48 pages)

Table of Contents

Safety

How to Obtain Services and Support

Introduction

Materials and Equipment

Notes about Micro Fluidic Card Experiments

Performing Reverse Transcription

Preparing and Running the Micro Fluidic Cards (PCR)

Performing Real-Time Data Analysis

Additional Tasks Related to Micro Fluidic Card Use

References

Appendix A Micro Fluidic Card Information CD

Index

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