Running Plates Using The Automation Controller Software; Loading Plates Onto The Plate Handler - Applied Biosystems 7900HT User Manual

Running Plates Using the Automation Controller Software

User Access
Requirement

Loading Plates onto the Plate Handler

Guidelines
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
There is no access requirement. All users can run plates that have been saved to the
SDS Enterprise Database.
Observe the following guidelines when loading plates onto the Plate Handler:
• Before loading plates onto the Plate Handler, make sure that for each plate:
– the associated plate document has been added to the plate queue or is
contained in the SDS Enterprise Database
– the plate has been sealed using an optical adhesive cover.
• Load the plates into the Plate Handler stacks in any order. The software reads
the bar code of each plate before it is run and matches the plate document and
method with the plate. The Automation Controller Software can run batches of
up to 84 plates in a single session (21 plates/stack).
• Orient the plates inside the stacks so that well A1 ( ) of each plate corresponds
to the locations shown in
• Do not place plates in the output stack (X). The Plate Handler arm uses the
empty stack to store plates after it runs them.
• If loading Low Density Array(s) onto the Plate Handler:
– Load no more than 20 cards per stack.
– Pack stacks of cards tightly. (The Plate Handler may fail to detect a
misaligned card correctly when loading it.)
Figure 4-11 Plate Positions of the Zymark Twister Microplate Handler

Running Plates Using the Automation Controller Software

Figure 4-11.
X
4
3
2
1
Bar code
Well A1
DRAFT
September 1, 2004 11:39 am, CH_Basic.fm
Zymark Twister Microplate
Handler (top view)
4-41