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1
Chapter 1 Getting started
About the QuantStudio
Data collection
14
6 and 7 Flex Real-Time PCR Systems
™
™
The QuantStudio
6 and 7 Flex Systems collect raw fluorescence data at different
points during a PCR, depending on the type of run that the QuantStudio
Flex Systems perform:
Table 1
Run type
Real-time
Standard curve
runs
Relative standard
curve
Comparative C
Melting curve
Post-PCR
Genotyping
(endpoint)
Presence/absence
runs
The instrument collects raw fluorescence data following each extension step of the
PCR. A data collection point, or read, on the QuantStudio
consists of three phases:
QuantStudio
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
™
Data collection point
The QuantStudio
data following each extension step of the PCR.
(ΔΔC
)
t
t
The QuantStudio
data:
1. Before the PCR. (For presence/absence
experiments, data collection before the
PCR is optional, but recommended.)
2. (Optional) During the PCR. The
QuantStudio
collect data during the run (real-time);
collecting data during the run can be
helpful for troubleshooting.
3. After the PCR.
™
6 and 7
™
6 and 7 Flex Systems collect
™
6 and 7 Flex Systems collect
™
6 and 7 Flex System can
™
6 and 7 Flex System
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