Centrifuging The Taqman Low Density Arrays - Applied Biosystems 7900HT User Manual

Chapter 4 Operating the Instrument

Centrifuging the TaqMan Low Density Arrays

Equipment and
Materials Needed
Placing Cards in
the Buckets
4-16 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, CH_Basic.fm
You need the following equipment and materials to centrifuge the Low Density
Arrays:
• TaqMan Low Density Array(s), containing sample-specific PCR reaction mix
(page 4-14)
• Sorvall ® Legend T Centrifuge
• Sorvall ® /Heraeus Custom Buckets (four required), each with its own card holder
• Blank balance cards
The centrifuge holds four Sorvall/Heraeus buckets. Each bucket holds up to
Note:
three Low Density Arrays (loaded and/or blank balance cards).
1. Obtain an empty Sorvall/Heraeus Custom Bucket and a card holder for the
centrifuge.
The Sorvall/Heraeus buckets and card holders required for the
IMPORTANT!
Low Density Arrays are custom-made. Do not use any other bucket/card holder
system for this procedure.
2. Place the bucket on a lab bench, with the label facing you.
3. Insert Low Density Arrays into the card holder, making sure that:
• The fill reservoirs project upwards out of the card holder
• The reaction wells face the same direction as the "This Side Out" label
Use blank balance cards to fill any remaining positions in the
IMPORTANT!
racks.
The card holder supports the Low Density Array fill reservoirs during
Note:
centrifugation.
4. Place a filled card holder in the bucket so that the "This Side Out" label faces
the front of the bucket, which has the Sorvall emblem on it.
Sorvall
emblem here
DRAFT