Before You Begin - Applied Biosystems 7900HT User Manual

Before You Begin

Using the SDS
Software
Online Help
Examples in
This Section
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
For specific instructions on any procedure described within this section, refer to the
Sequence Detection Systems Software Online Help. To get help at any time during the
procedure, click located within the dialog box or window in which you are
working.
The illustrations and screenshots that appear within this chapter were created from a
plate containing Pre-Developed TaqMan Assays and Reagents for Allelic
Discrimination run to screen 6 human genomic DNA samples (HD 1, 2, 3, 4, 7, 8) for
2 targets (CYP 2C9*2 and CYP 2C19*2). Each well of the plate contains 1 µL DNA,
TaqMan ® 2✕ Universal PCR Master Mix, forward and reverse primers, and FAM and
VIC dye-labeled TaqMan probes.
Figure 5-5 illustrates the arrangement of the assays, unknown samples, and no
template control (NTC) wells on the plate.
HD-1 HD-1 HD-1
HD-2 HD-2 HD-2
HD-3 HD-3 HD-3
HD-4 HD-4 HD-4
HD-7 HD-7 HD-7
HD-8 HD-8 HD-8
PDAR CYP 2C9*2
PDAR CYP 2C19*2
Figure 5-5 Configuration of the Samples and Detectors on the Example Plate
Note: The probes used in the example experiment were designed using the Primer
Express ® Primer Design Software and by following the guidelines explained in
"Assay Development Guidelines" on page
The SDS software does not require that absolute quantification plates
IMPORTANT!
contain positive controls.
HD-1 HD-1 HD-1 HD-1
HD-2 HD-2 HD-2 HD-2
HD-3 HD-3 HD-3 HD-3
HD-4 HD-4 HD-4 HD-4
HD-7 HD-7 HD-7 HD-7
HD-8 HD-8 HD-8 HD-8
Not in Use
B-2.
September 1, 2004 11:39 am, CH_End-Point.fm

Before You Begin

HD-1 HD-1 HD-1
HD-2 HD-2 HD-2
HD-3 HD-3 HD-3
NTC
HD-4 HD-4 HD-4
HD-7 HD-7 HD-7
HD-8 HD-9 HD-8
DRAFT
5-9