Applied Biosystems GeneAmp PCR System 9700 User Manual
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Applied Biosystems GeneAmp PCR System 9700 User Manual

Applied Biosystems GeneAmp PCR System 9700 User Manual

Dual 96 sample block module
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GeneAmp® PCR System 9700
Auto-Lid Dual 96 and Dual 96 Sample Block
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User Guide
GeneAmp® PCR System 9700
Dual 96 Sample Block Module
User Guide

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Summary of Contents for Applied Biosystems GeneAmp PCR System 9700

  • Page 1 GeneAmp® PCR System 9700 Auto-Lid Dual 96 and Dual 96 Sample Block Module User Guide GeneAmp® PCR System 9700 Dual 96 Sample Block Module User Guide...
  • Page 2 Information in this document is subject to change without notice. Applied Biosystems assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Applied Biosystems be liable for incidental, special, multiple, or consequential damages in connection with or arising from the use of this document.

  • Page 3: Table Of Contents

    ......... 2-3 GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide...
  • Page 4 ....... 3-15 Index GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide...

  • Page 5: Preface

    You must create a separate Sample Entry Spreadsheet for each 96-well IMPORTANT! microtiter plate. Safety Alert Safety alert words also appear in user documentation. For more information, see “Safety Alert Words” page viii. Words GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide...

  • Page 6: How To Obtain More Information

    • Download software updates and patches In addition, the Services and Support page provides access to worldwide telephone and fax numbers to contact Applied Biosystems Technical Support and Sales facilities. GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide...
  • Page 7 ..........xv Safety and Electromagnetic Compatibility (EMC) Standards ....xvi GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide...

  • Page 8: Safety And Emc Compliance Information

    Safety and EMC Compliance Information Safety Conventions Used in This Document Safety Alert Four safety alert words appear in Applied Biosystems user documentation at points in the document where you need to be aware of relevant hazards. Each alert Words word–IMPORTANT, CAUTION, WARNING, DANGER–implies a particular...

  • Page 9: Symbols On Instruments

    Indicates the presence of an electrical shock hazard and to proceed with appropriate caution. Indicates the presence of a hot surface or other high-temperature hazard and to proceed with appropriate caution. GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide...

  • Page 10: Safety Labels On Instruments

    Applied Biosystems qualified service pièce réparable par l’utilisateur. Toute personnel. intervention doit être effectuée par le personnel de service qualifié de Applied Biosystems. CAUTION Moving parts. ATTENTION Parties mobiles. GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide...

  • Page 11: General Instrument Safety

    PHYSICAL INJURY HAZARD. Use this product only as specified in this document. Using this instrument in a manner not specified by Applied Biosystems may result in personal injury or damage to the instrument. Moving and PHYSICAL INJURY HAZARD. The instrument is to be moved...
  • Page 12 Safety and EMC Compliance Information Obtaining You can obtain from Applied Biosystems the MSDS for any chemical supplied by Applied Biosystems. This service is free and available 24 hours a day. MSDSs To obtain MSDSs: 1. Go to https://docs.appliedbiosystems.com/msdssearch.html 2. In the Search field, type in the chemical name, part number, or other information that appears in the MSDS of interest.

  • Page 13: Chemical Waste Safety

    • Ensure that the instrument waste is stored, transferred, transported, and disposed of according to all local, state/provincial, and/or national regulations. Radioactive or biohazardous materials may require special handling, IMPORTANT! and disposal limitations may apply. GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide xiii...

  • Page 14: Physical Hazard Safety

    108 °C, and the temperature of the sample block can e as high as 100 °C. Before performing the procedure, keep hands away until the heated cover and sample block reach room temperature. GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide...

  • Page 15: Workstation Safety

    • Use equipment that comfortably supports you in neutral working positions and allows adequate accessibility to the keyboard, monitor, and mouse. • Position the keyboard, mouse, and monitor to promote relaxed body and head postures. GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide...

  • Page 16: Safety And Electromagnetic Compatibility (Emc) Standards

    This instrument has been tested to and complies with standard AS/NZS 2064, “Limits and Methods Measurement of Electromagnetic Disturbance Characteristics Standards of Industrial, Scientific, and Medical (ISM) Radio-frequency Equipment.” GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide...

  • Page 17: Dual 96 Sample Block Module Overview

    ..........1-9 DRAFT GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide October 24, 2007 9:40 am, 44343363 D 03 Overview.fm...

  • Page 18: About The Dual 96 Sample Block Module

    To upgrade the base module software, use the floppy disk that is shipped with the Dual 96 Sample Block Module. For instructions on upgrading the software, see the GeneAmp PCR System 9700 Base Module User’s Manual (P/N 4303481) or see “How to Obtain Services and...
  • Page 19 If you use just one reaction plate, place it on Using reaction plates on both sides the right. Place an empty plate on the left. DRAFT GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide October 24, 2007 9:40 am, 44343363 D 03 Overview.fm...
  • Page 20 • Uniformity of combined sample blocks: ±0.75 °C (measured after 30 sec at 95 °C) • Long-term stability and high reliability Modules, The modules listed below are available for the GeneAmp PCR System 9700 Base Module. The accessories and disposables are for use with the Dual 96 Sample Block Accessories, and Module.
  • Page 21 Reaction Tubes with Caps N801-0540 ® MicroAmp 96-Well Tray for Tubes with Caps N801-0541 ® MicroAmp Centrifuge Adapter N801-3822 DRAFT GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide October 24, 2007 9:40 am, 44343363 D 03 Overview.fm...

  • Page 22: Installing The Dual 96 Sample Block Module

    Figure 1-2 Top view, Dual 96 Sample Block Module Lever Figure 1-3 Bottom view, Dual 96 Sample Block Module Installing the To install the sample block module into the GeneAmp PCR System 9700 Base Module: Sample Block Module 1. Pull out the lever from the sample block module.

  • Page 23: Sample Tray And Plate Configurations

    MicroAmp 96-Well Support Base MicroAmp 8-Strip Caps MicroAmp Caps, 8-Strip MicroAmp Optical 96-Well Reaction Plate MicroAmp 96-Well Support Base DRAFT GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide October 24, 2007 9:40 am, 44343363 D 03 Overview.fm...
  • Page 24 Full Plate Cover Cover MicroAmp 96-Well Retainer MicroAmp 8-Strip Tubes or Single Tubes MicroAmp 96-Well Tray MicroAmp 96-Well Support Base DRAFT GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide October 24, 2007 9:40 am, 44343363 D 03 Overview.fm...

  • Page 25: Loading Samples

    4. Pipette the samples into the tubes. 5. Cap the tubes using either the MicroAmp 96-Well Full Plate Cover or the MicroAmp 8-Strip Caps. DRAFT GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide October 24, 2007 9:40 am, 44343363 D 03 Overview.fm...
  • Page 26 Do not open the cover when the block temperature is above 27 °C IMPORTANT! because sample caps may pop off or jam. DRAFT 1-10 GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide October 24, 2007 9:40 am, 44343363 D 03 Overview.fm...

  • Page 27: Maintaining The Sample Block Module

    ........2-3 GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide...

  • Page 28: Cleaning The Sample Block Module

    • Clean the heated cover • Clean the sample block wells Before using any cleaning or decontamination method, except those recommended in this manual, check with Applied Biosystems to ensure that the proposed method will not damage the equipment. Required...

  • Page 29: Cleaning The Heated Cover

    3. Remove any remaining isopropanol from the heated cover with a clean cloth and return the cover to its normal position. Cleaning the Sample Block Wells Clean the sample block wells once a month or more frequently, if needed. GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide...
  • Page 30 Please read the MSDS, and follow the handling instructions. Wear appropriate protective eyewear, clothing, and gloves. GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide...

  • Page 31: Performing The Calibration Verification Test

    Performing System Performance Diagnostics......3-15 GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide...
  • Page 32 This test requires the Temperature Verification System, Dual 0.2 mL (P/N 4346117), which includes: Materials • Light mineral oil • Cotton swabs • Temperature Verification Plates • 0.2-mL Probe Assembly • Digital thermometer with 9 V battery installed GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide...
  • Page 33 3. Press F1 (Diag). The Diagnostics screen is displayed. 4. Press F3 (TmpVer). The Temperature Verification screen is displayed. Temperature Verification Temp - Calibration Verification TNU - Temperature Non-Uniformity Temp Exit GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide...
  • Page 34 2. When the “Stabilizing at setpoint” value decrements to zero, read the digital thermometer. Refer to the instructions included with the Temperature Verification System for a detailed description on operating the digital thermometer. GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide...
  • Page 35 6. If the values agree, press F1 (Accept). 7. Continue with “Evaluating the Results” on page 3-6 “Ending the Test” on page 3-6. GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide...
  • Page 36 4. Wait for the sample block to reach room temperature (~25 °C), then remove the 96-Well Temperature Verification Plates from the sample blocks. 5. Clean the oil from the sample block. GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide...
  • Page 37 Use a photocopy of this page to record values, and keep the original IMPORTANT! intact. Setpoint Value: Well R3 Probe Thermometer Date Tested By Serial No. Serial No. 85 °C 45 °C GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide...

  • Page 38: Performing The Temperature Non-Uniformity Test

    Chapter 3 Testing the Dual 96 Sample Block Module Performing the Temperature Non-Uniformity Test Overview Use this test to find wells within the GeneAmp PCR System 9700 Dual 96-Well Sample Block Module that exhibit unacceptable temperature variation. To complete the Temperature Non-Uniformity Test, you perform the following tasks,...
  • Page 39 “Setting Up the 0.2-mL Probe Assembly” on page 3-8 Test – “Configuring the System 9700” on page 3-9 • Obtain the current Temperature Uniformity Data Sheet for your system, or copy the form on page 3-14. GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide...
  • Page 40 2. When the “Stabilizing at setpoint” value decrements to zero, read the digital thermometer. Refer to the instructions included with the Temperature Verification System for a detailed description on operating the digital thermometer. 3-10 GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide...
  • Page 41 The TNU Performance screen is displayed with the following message: TNU Performance Sample temp = xx.x°C Cover temp = xxx°C Place probe in well xx Press Run Cancel GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide 3-11...
  • Page 42 11. If the values agree, press F1 (Accept). 12. Continue with “Evaluating the Results” on page 3-13 “Ending the Test” on page 3-13. 3-12 GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide...
  • Page 43 Biosystems Technical Support. See “How to Obtain Services and Support” page Ending the Test To end the test, follow the procedures in “Ending the Test” on page 3-6. GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide 3-13...
  • Page 44 Use a photocopy of this page to record values, and keep the original IMPORTANT! intact. Date Tested By Probe Serial No. Meter Serial No. Setpoint Value 95 °C 55 °C 3-14 GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide...
  • Page 45 Performing System Performance Diagnostics Performing System Performance Diagnostics After you have configured the GeneAmp PCR System 9700, conduct the system Rate and Cycle performance tests to verify the integrity of the cooling and heating system. Before you begin these tests, make sure that you place an empty...
  • Page 46 Average Cycle Time: xxx.x sec x.x sec Cycle Time STD: Print Cancel The following table lists the passing ranges for the Cycle Test. Average Cycle Time <= 160 sec. 3-16 GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide...
  • Page 47 Performing System Performance Diagnostics Cycle Time STD < 5 sec. GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide 3-17...
  • Page 48 Chapter 3 Testing the Dual 96 Sample Block Module 3-18 GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide...
  • Page 49 Dual 96 Sample Block Module Temperature Verification Kit, part number chemical waste disposal, guidelines xiii cleaning the sample block module 2-2, conventions bold text electrical hazard symbol IMPORTANTS! electrical safety in this guide GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide Index-1...
  • Page 50 DNA contamination part numbers heated lid parts, ordering heating and cooling, information Peltier unit heating/cooling unit verifying running correctly 3-15 IMPORTANT performance tests 3-15, 3-16 description v, viii Index-2 GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide...
  • Page 51 1-10 verifying temperature uniformity 3-13 US safety standards sample compartment, loading samples 1-10 user attention words, defined GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide Index-3...
  • Page 52 WARNING, description viii waste disposal, guidelines xiii workstation safety Index-4 GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide...
  • Page 53 Toll Free (In North America): +1 800.345.5224 Fax: +1 650.638.5884 Worldwide Sales and Support Applied Biosystems vast distribution and service network, composed of highly trained support and applications personnel, reaches 150 countries on six continents. For sales office locations and technical support, please call our local office or refer to our Web site at www.appliedbiosystems.com.

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