Preparing The Pure Dye Plates Or Cards; Preparing A Plate Document For A Pure Dye Plate Or Card - Applied Biosystems 7900HT User Manual

Chapter 7 Maintaining the Instrument

Preparing the Pure Dye Plates or Cards

Preparing the
Pure Dye Plates
Preparing the
Microfluidic Pure
Dye Cards

Preparing a Plate Document for a Pure Dye Plate or Card

Preparing a
Pure Dye Plate
Document
7-22 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, CH_Maintenance.fm
A background run must be performed before running a pure dye plate.
IMPORTANT!
See "Performing a Background Run" on page 7-16
1. Remove the pure dye plate from the refrigerator or freezer and set it aside to
warm to room temperature.
2. Remove the pure dye plate from its packaging.
3. Briefly centrifuge the pure dye plate.
A background run must be performed before running the microfluidic
IMPORTANT!
pure dye cards. See "Performing a Background Run" on page 7-16
information.
1. Remove the three empty microfluidic pure dye cards from the refrigerator and
set them aside to warm to room temperature.
2. Remove the three dye tubes (FAM, VIC, and ROX dyes) from the freezer to
thaw. When the dyes have thawed, vortex the tubes.
3. Load the FAM dye into one of the empty microfluidic pure dye cards, loading
100 µL of dye per fill reservoir.
4. Centrifuge the microfluidic pure dye card per the procedures in
the TaqMan Low Density Arrays" on page
5. Seal the microfluidic pure dye card per the procedures in
Low Density Arrays" on page
6. Repeat steps 3 through 5
1. Start the SDS software.
2. Click (or select File > New).
3. Configure the New Document dialog box:
Assay – Select Pure Spectra.
Container – Select the appropriate format.
Template – If performing a:
• Standard 384-Well Pure Dye Run: select 384 Well Pure Dyes Plate.sdt.
If no plate document templates are available, construct a Pure Dye plate
document using the product insert from the Spectral Calibration Kit and
the procedure on
• Standard 96-Well Pure Dye Run: select the plate document template
matching the Pure Dye plate you intend to run.
To run Plate 1 (containing FAM, JOE
96 Well Pure Dyes Plate 1.sdt.
To run Plate 2 (containing SYBR, TAMRA, TET, and VIC dyes), select
96 Well Pure Dyes Plate 2.sdt.
DRAFT
4-16.
4-19.
for the VIC and ROX dyes.
page 7-28.
™ , NED, and ROX dyes), select
for more information.
for more
"Centrifuging
"Sealing the TaqMan