Opening The Run Data; Analyzing The Run Data - Applied Biosystems 7900HT User Manual

Opening the Run Data

User Access
Requirement
Open the
Un-analyzed
Plate Document

Analyzing the Run Data

User Access
Requirement
Analyzing the
Run
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
There is no access requirement. All users can open dissociation curve data that has
been saved to the SDS Enterprise Database.
Opening a Plate Document File
1. Click (or select File > Open).
2. In the Look in field, navigate to and select the plate document file.
3. Click .
The SDS software displays the plate document file.
4. Configure the Analysis Settings for each marker as explained below.
Opening Un-analyzed Data from the SDS Enterprise Database
5. Click (or select File > Open Document from Database).
6. In the Plate Query dialog box, configure the Find Plates Matching These
Criteria table with parameters that correspond to the session of interest.
7. Click .
8. In the Search Results list, scroll to and select the session that you want to
analyze.
9. Click .
10. Click when finished.
There is no access requirement. All users can analyze dissociation curve data that has
been saved to the SDS Enterprise Database.
The run data from a temperature ramp can be analyzed immediately following the
completion of the run. For an explanation of how the software manipulates the raw
data, see "Algorithmic Manipulation of Raw Data" on page
1. Select all wells in the plate grid.
The software outlines the selected wells with a black border.
2. Choose one of the following:
• Select Analysis > Analyze.
• Click .
The software analyzes the run data and displays the results in the Dissociation
Curve tab.
3. Determine the T
m
Curve tab as explained on
for the dissociation curves displayed within the Dissociation
page 6-44.
September 1, 2004 11:39 am, CH_Real-Time.fm

Opening the Run Data

6-7.
DRAFT
6-41