Chapter 8 Troubleshooting; Troubleshooting Table - Applied Biosystems 7900HT User Manual

Chapter 8 Troubleshooting

Troubleshooting Table

Overview
8-2 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, CH_Trouble.fm
The following table is designed to help you troubleshoot most of the problems you
may encounter while using the Applied Biosystems 7900HT Fast Real-Time PCR
System.
The information in the table is arranged by category as follows:
• Chemistry problems
• Run problems
• Instrument and Automation Accessory Problems
Each category contains subcategories, followed by a brief description of the
symptoms you might encounter.
To use this table, look for the category and the symptom you are experiencing. The
page number in the right-hand column corresponds to a description of the possible
cause(s) and recommended action(s) for that particular problem.
Table 8-1 Troubleshooting Table
Category
Chemistry and Run Problems
Chemistry
Software Installation
SDS Software
Run Problems
Background Runs
Pure Dye Runs
Real-Time Runs (Quantitative PCR and Dissociation Curves)
End-Point Runs (Allelic Discrimination)
DRAFT
Symptom
Low Precision
Irreproducibility
Unable to finish installation
(Install program appears to b e frozen)
Installation Interrupted
Software will not extract background data
Background is too high (greater than 2500)
Software will not extract pure dye data
Raw data from pure dye run appears strange
Signals plateau (saturation)
Signal is too low (< 10,000 FSU)
More than two outliers per dye in a single row
Page
8-5
8-5
8-9
8-11
8-12
8-13