Sign In
Upload
Manuals
Brands
Applied Biosystems Manuals
Laboratory Equipment
7300
Applied Biosystems 7300 Manuals
Manuals and User Guides for Applied Biosystems 7300. We have
3
Applied Biosystems 7300 manuals available for free PDF download: Installation And Maintenance Manual, Getting Started Manual
Applied Biosystems 7300 Installation And Maintenance Manual (174 pages)
Real-Time PCR System
Brand:
Applied Biosystems
| Category:
Laboratory Equipment
| Size: 15.12 MB
Table of Contents
7300/7500/7500 Fast System
5
Installation and Maintenance Workflow
5
Table of Contents
7
How to Use this Guide
11
Preface
11
How to Obtain more Information
12
How to Obtain Support
13
Safety and EMC Compliance Information
16
Safety Conventions Used in this Document
16
Symbols on Instruments
17
Safety Labels on Instruments
18
General Instrument Safety
20
Chemical Safety
21
Chemical Waste Safety
22
Electrical Safety
23
Biological Hazard Safety
24
Physical Hazard Safety
24
Safety and Electromagnetic Compatibility (EMC) Standards
25
Workstation Safety
25
Before Starting the Installation
27
Chapter 1 Before Starting the Installation
28
Selecting the Installation Method
28
Setting up the Computer
28
Planning the Installation
31
Unpacking and Setting up the Instrument
33
Before You Begin
34
Chapter 2 Unpacking and Setting up the Instrument
34
Selecting Additional Hardware and Software
35
Uncrating and Placing the 7300/7500/7500 Fast System
38
Checking the Shipped Materials
40
Setting up the 7300/7500/7500 Fast Instrument
46
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
47
Installing the Software and Powering on the Instrument
49
Chapter 3 Installing the Software and Powering on the Instrument
50
Overview
50
Installing the SDS Software for the 7300/7500/7500 Fast System
51
Powering on the Instrument
54
Chapter 4 Performing the Instrument Function Test
56
Troubleshooting
58
Performing the Regions of Interest (ROI) Calibration
61
Overview
62
Preparing the ROI Calibration Plate
64
Creating a Plate Document for the ROI Calibration
67
Performing the ROI Calibration
70
Troubleshooting
74
Performing the Background Calibration and Optical Calibration
75
Background Calibration and Optical Calibration
76
Overview
76
Preparing the Background Calibration Plate
78
Creating a Plate Document for the Background Calibration
80
Performing the Background Calibration
81
Analyzing the Background Calibration Data
82
Analyzing the Background Calibration Data
83
Performing the Optical Calibration for the 7500/7500 Fast System
85
Troubleshooting
90
Performing the Pure Dye Calibration
93
Chapter 6 Performing the Pure Dye Calibration
94
Overview
94
Preparing for the Calibration
97
Creating a Plate Document for the Pure Dye Calibration
98
Preparing Plates and Performing the Pure Dye Calibration
99
Analyzing the Pure Dye Calibration Data
103
Evaluating Pure Dye Calibration Spectra
106
Verifying the Instrument Performance
113
Chapter 7 Verifying the Instrument Performance
114
Overview
114
Preparing the Taqman Rnase P Verification Plate
116
Creating a Plate Document for the Rnase P Verification Run
118
Chapter 5 Performing the Verification Run
119
Analyzing the Taqman Rnase P Plate Data
120
Troubleshooting
125
Powering off the 7300/7500/7500 Fast System
127
Performing a Short-Term Shutdown
128
Performing a Long-Term Shutdown
130
Appendix A Maintaining the Instrument
131
Maintaining the Instrument
131
Recommended Maintenance Schedule
131
Recommended Maintenance Schedule
132
Archiving and Backing up SDS Files
133
Decontaminating the Sample Block
134
Cleaning up and Defragmenting the Hard Drive
140
Defragmenting the Hard Drive
140
Moving the 7300/7500/7500 Fast System
141
Monitoring Lamp Status
143
Replacing the Halogen Lamp
144
Replacing the Instrument Fuses
148
Updating the Operating System Software and Service Packs
150
Appendix B Creating a Background Plate
151
Creating a Background Plate
151
Overview
151
Appendix C Creating a Custom Pure Dye Plate
153
Overview
153
Determining Optimum Dye Concentration
154
Creating a Custom Pure Dye Plate
156
Adding the Custom Dye to the Software
157
Setting up the Computer
159
Appendix D Setting up the Computer
160
Unpacking the Computer and Setting Voltage
160
Connecting Components and Powering up
162
Setting the Display Settings and Power Options
163
Connecting to the Network and Downloading Adobe Acrobat
166
Index
167
Advertisement
Applied Biosystems 7300 Getting Started Manual (106 pages)
Fast Real-Time PCR System
Brand:
Applied Biosystems
| Category:
Laboratory Equipment
| Size: 4.29 MB
Table of Contents
Relative Quantification Experiment Workflow
3
Table of Contents
5
How to Use this Guide
7
Preface
7
How to Obtain more Information
9
How to Obtain Support
10
Introduction
11
About Relative Quantification
12
About the 7300/7500/7500 Fast System
12
Chapter 1 Introduction
12
About RQ Experiments
13
Designing an RQ Experiment
17
Selecting the PCR Method
18
Chapter 2 Designing an RQ Experiment
18
Specifying the Components of an RQ Experiment
19
Selecting the Chemistry
21
Selecting One- or Two-Step RT-PCR
22
Choosing the Probes and Primers
24
Performing Reverse Transcription
27
Guidelines for Preparing RNA
28
Chapter 3 Performing Reverse Transcription
28
Converting Total RNA to Cdna
29
Chapter 4 Generating Data from RQ Plates - 7300 or Standard 7500 System
31
Before You Begin
32
Preparing the PCR Master MIX
32
Preparing the Reaction Plate
33
Creating a Relative Quantification (RQ) Plate Document
35
Specifying Thermal Cycling Conditions and Starting the Run
40
Analyzing and Viewing RQ Plate Data
43
Exporting RQ Plate Data
46
Chapter 5 Generating Data from RQ Plates - 7500 Fast System
47
Before You Begin
48
Preparing the PCR Master MIX
48
Preparing the Reaction Plate
50
Creating a Relative Quantification (RQ) Plate Document
53
Specifying Thermal Cycling Conditions and Starting the Run
58
Analyzing and Viewing RQ Plate Data
62
Exporting RQ Plate Data
65
Troubleshooting
66
Analyzing Data in an RQ Study
69
Creating an RQ Study Document
70
Chapter 6 Analyzing Data in an RQ Study
70
Configuring Analysis Settings
73
Adjusting the Baseline and Threshold
75
Analyzing and Viewing the Results of the RQ Study
80
Reanalyzing an RQ Study
84
Omitting Samples from a Study
85
Exporting RQ Study Data
88
Appendix A Creating Detectors
89
Appendix B Example RQ Experiment
92
References
100
Index
102
Applied Biosystems 7300 Getting Started Manual (78 pages)
Fast Real-Time PCR System
Brand:
Applied Biosystems
| Category:
Laboratory Equipment
| Size: 5.59 MB
Table of Contents
Plus/Minus Experiment Workflow
3
Table of Contents
5
How to Use this Guide
7
Preface
7
How to Obtain more Information
9
How to Obtain Support
10
Introduction
11
About Plus/Minus Assays Using an IPC
12
About the 7300/7500/7500 Fast System
12
Chapter 1 Introduction
12
About Plus/Minus Assays
14
Designing a Plus/Minus Experiment
17
Using Taqman ® Probe-Based Reagent Configuration
18
Chapter 2 Designing a Plus/Minus Experiment
18
Designing the Probe and Primers
19
Setting up the Reaction Plate
21
Preparing DNA
22
Chapter 3 Setting up the Reaction Plate
22
Setting up the Reaction Plate
23
Performing the Plus/Minus Pre-Read Run
27
The Pre-Read Run
28
Before You Begin
28
Creating a Plus/Minus Plate Document
28
Chapter 4 Performing the Plus/Minus Pre-Read Run
28
Performing the Pre-Read Run
34
Performing the Pre-Read Run
35
Generating Amplification Data
37
Creating a Plate Document for Sample Amplification
38
Chapter 5 Generating Amplification Data
38
Performing the Amplification Run
42
Chapter 6 Performing the Plus/Minus Post-Read Run
46
Performing the Post-Read Run
47
Viewing Plus/Minus Results
50
Exporting Plus/Minus Plate Data
54
Appendix A Creating Detectors
56
Appendix B Viewing Amplification Data
58
Viewing the Amplification Data
60
Appendix C Example Plus/Minus Experiment
66
References
72
Index
74
Advertisement
Advertisement
Related Products
Applied Biosystems 7900HT
Applied Biosystems 7500
Applied Biosystems 7500 Fast
Applied Biosystems QuantStudio 7 Flex
Applied Biosystems 2720
Applied Biosystems A30207
Applied Biosystems A30208
Applied Biosystems A31486
Applied Biosystems A31487
Applied Biosystems A31488
Applied Biosystems Categories
Laboratory Equipment
Measuring Instruments
Bicycle Accessories
Software
Control Unit
More Applied Biosystems Manuals
Login
Sign In
OR
Sign in with Facebook
Sign in with Google
Upload manual
Upload from disk
Upload from URL