Chapter 5 Analyzing End-Point Data; End-Point Runs On The 7900Ht Instrument - Applied Biosystems 7900HT User Manual

Chapter 5 Analyzing End-Point Data

End-Point Runs on the 7900HT Instrument

End-Point Runs
5-2 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, CH_End-Point.fm
End-point is the term used to describe the category of sequence detection runs in
which the Applied Biosystems 7900HT Fast Real-Time PCR System is used to
measure the fluorescence of a biological sample after it has undergone thermal
cycling. Unlike real-time runs that can yield quantitative measurements, the focus of
end-point experiments is typically to produce a qualitative result. End-point analysis
is commonly used in combination with TaqMan
the presence or absence of specific target nucleic acid sequence in cells, tissues, or
fluid samples.
Currently, the SDS software supports one type of end-point analysis: Allelic
Discrimination.
DRAFT
® reagent-based chemistry to confirm