Applied Biosystems 7900HT User Manual page 210

Chapter 6 Analyzing Real-Time Data
Table 6-1 Summary of Expression Levels
Sample ∆C
Detector
Calibrator
a
Target <
Endo <
b
Target Max
Endo <
Target <
Endo <
Target Max
Endo Max
Target <
Endo Max
Target Max
Endo Max
Target Max
Endo Max
Target Max
Endo <
Target <
Endo Max
Target <
Endo Max
Target <
Endo <
Target Max
Endo Max
Target Max
Endo <
Target Max
Endo <
Target <
Endo <
Target <
Endo Max
a< : Indicates that the sample C
bMax (or —): Indicates that the amplification curves for the associated sample replicate group never crossed the threshold
during the duration of the run ('undetermined' values).
6-32 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, CH_Real-Time.fm
s
T
Then expression
Test
<
<
<
(bar displays ↑)
<
Max
(bar displays ↓ )
<
Max Undetermined
Max (not displayed)
Max Undetermined
Max (not displayed)
< Undetermined
Max (not displayed)
Max Undetermined
< (not displayed)
Max Undetermined
Max (not displayed)
< Undetermined
Max (not displayed)
Max Undetermined
< (not displayed)
Max Undetermined
Max (not displayed)
< Undetermined
< (not displayed)
Max Undetermined
< (not displayed)
< Undetermined
Max (not displayed)
< Undetermined
Max (not displayed)
< Undetermined
< (not displayed)
is less than the maximum PCR cycle of the run (a valid C
T
DRAFT
Example (for a 40-cycle experiment)
level is...
Calib ∆C
Displayed 25
33
b
A minimum —
35
A maximum 33
33
—
—
30
—
—
—
—
—
—
30
30
—
35
—
30
20
—
—
—
20
—
33
30
35
33
—
Test ∆C
Result
T T
32 • RQ is displayed
• Bar is displayed
35
34 • RQ is displayed
• Bar displays ↑
30
— • RQ is displayed
• Bar displays ↓
33
— • RQ value is not
calculated
—
• Bar is not displayed
—
—
30
—
—
30
—
—
20
—
—
33
—
—
30
20
—
20
35
—
33
—
30
35
).
T