Applied Biosystems 7900HT User Manual page 101

Assigning
Standards for
Absolute
Quantification
Selected wells
(quantity applied
to selection)
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
3. Repeat steps 1 through 2
4. Choose from the following options:
If constructing a
plate document for...
absolute quantification
• allelic discrimination
• relative quantification
• dissociation curve analysis
For the SDS software to create a standard curve for quantification of unknown
samples, you must apply the Standard task and quantity values to your absolute
quantification plate document.
1. In the plate grid of the SDS software, select the replicate wells containing the
first standard in the dilution series.
2. In the well inspector, click the field in the Task column for the appropriate
detector entry, and select the Standard from the drop-down list.
3. In the well inspector, click the field in the Quantity column for the appropriate
detector, enter a quantity or concentration for the standard in the appropriate
unit of measurement (starting copy number, picograms, nanograms, etc.), and
press Enter.
The software labels the selected standard wells with the specified quantity.
4. Repeat steps 1 through 3
standard wells on the plate.
When finished, the plate grid should contain a complete set of replicate wells
labeled with the Standard task and assigned quantities that the software will
use to compute the standard curve for the run.
5. Do one of the following:
• If necessary, set the passive reference for the plate document as explained on
"Step 4 – Setting the Passive Reference and Omitting Wells" on page
• Otherwise, program the method for your run as explained in
"Step 5 – Programming the Plate Document Method" on page
Step 3 – Configuring the Plate Document with Tasks
to apply any remaining tasks to the plate document.
Then...
assign quantities to the standard wells of the plate
document as explained below.
• If necessary, set the passive reference for the
plate document as explained on
• Otherwise, program the method for your run as
explained on page
Quantity field
to configure the plate with the other sets of replicate
DRAFT
September 1, 2004 11:39 am, CH_Run.fm
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