Markers List (Allelic Discrimination Only - Applied Biosystems 7900HT User Manual

9. Markers List (Allelic Discrimination Only)

Description
Format (for a
single marker):
Examples:
Example Code A-5 Allelic discrimination setup table file
*** SDS Setup File Version
*** Output Plate Size
*** Output Plate ID
*** Number of Detectors
Detector Reporter
CYP 2C9*2.1 FAM
CYP 2C9*2.2 VIC
*** Number of Markers
Marker AlleleX
CYP 2C9*2 CYP 2C9*2.2
...
Example Code A-6 Absolute or relative quantification setup table file
*** SDS Setup File Version
*** Output Plate Size
*** Output Plate ID
*** Number of Detectors
Detector Reporter
GAPDH VIC
SYBR Green SYBR
RNase P FAM
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
Marker List information is required only in setup table files created
IMPORTANT!
for allelic discrimination runs. This section does not appear in setup table files
created for absolute or relative quantification runs.
The marker list consists of one or more lines displaying the information for each
marker used on the plate document.
• The Markers List section must contain one line (or definition) for each marker
present on the plate.
• The number of lines in the Markers List section must be equal to the number
defined in the Number of Markers section (see number 7 above).
• Leave the markers list line blank in setup files generated for relative and
absolute quantification. (Real-time plate documents do not use markers.)
[ marker name <tab> alleleX <tab> alleleY <tab> description <tab>
comments <cr> ]
3
384
384N75822034
2
Quencher
1
AlleleY
CYP 2C9*2.2
3
96
96JG729FF903
3
Quencher
TAMRA
Description
PDAR CYP 2C9*2 Al1
PDAR CYP 2C9*2 Al2
Description
PDAR CYP 2C9*2
Description
GAPDH Probe
SYBR Green I
RNase P Probe
September 1, 2004 11:39 am, App_SWReference.fm
Setup Table Files
Comments
Example Probe
Example Probe
Comments
Example marker
Comments
Example Probe
Example Probe
Example Probe
DRAFT
A-9