Applied Biosystems GeneAmp 2720 User Manual
  1. Manuals
  2. Brands
  3. Applied Biosystems Manuals
  4. Laboratory Equipment
  5. 2720
  6. User manual

Applied Biosystems GeneAmp 2720 User Manual

Thermal cycler temperature verification system
Hide thumbs Also See for GeneAmp 2720:
Table of Contents

Advertisement

Quick Links

Download this manual
Thermal Cycler Temperature
Verification System
For GeneAmp
®
PCR System 2720, 9600, 9700, 9800, and
Veriti
Thermal Cyclers
®
User Guide

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the GeneAmp 2720 and is the answer not in the manual?

Questions and answers

Related Manuals for Applied Biosystems GeneAmp 2720

Summary of Contents for Applied Biosystems GeneAmp 2720

  • Page 1 Thermal Cycler Temperature Verification System For GeneAmp ® PCR System 2720, 9600, 9700, 9800, and Veriti Thermal Cyclers ® User Guide...
  • Page 2 © Copyright 2009, Life Technologies Corporation. All rights reserved. Information in this document is subject to change without notice. Applied Biosystems assumes no responsibility for any errors that may appear in this document. APPLIED BIOSYSTEMS DISCLAIMS ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE.

  • Page 3: Table Of Contents

    Contents Preface Safety information ............vii How to use this guide .
  • Page 4 Data Sheet: Temperature Non-Uniformity Test ....... . 48 ® Temperature Verification Tests for the GeneAmp 9700, Dual 384-Well Sample Block Module Calibration verification test .
  • Page 5 ® Temperature Verification Tests for the Veriti Thermal Cycler Overview ............118 Calibration verification test .
  • Page 6 ® Thermal Cycler Temperature Verification System For GeneAmp PCR Systems 2720, 9600, 9700, 9800, ® and Veriti System User Guide...

  • Page 7: Preface

    “Safety” Appendix for the complete alert on the chemical or instrument. Safety alert Four safety alert words appear in Applied Biosystems user documentation at points words in the document where you need to be aware of relevant hazards. Each alert word—IMPORTANT, CAUTION, WARNING, DANGER—implies a particular...

  • Page 8: How To Use This Guide

    • A right arrow symbol ( ) separates successive commands you select from a drop-down or shortcut menu. For example: Select File Open Spot Set. User attention Two user attention words appear in Applied Biosystems user documentation. Each words word implies a particular level of observation or action as described below: Note: –...

  • Page 9: Introduction

    Introduction This chapter covers: About this manual ........... .2 About the Temperature Verification System .

  • Page 10: About This Manual

    PCR System 9700, Dual 384-Well Sample Block Module ® • GeneAmp PCR System 9800 ® • Applied Biosystems Veriti 96-Well Fast Thermal Cycler This manual contains a separate chapter for each instrument. Each instrument chapter provides step-by-step instructions for performing two different tests, which are described in “Temperature verification tests”...
  • Page 11 Chapter 1 About this manual Digital thermometer 4500 ® Frame for GeneAmp 9700, 9600, and 2720 thermal cycler MODE GR1918 Probe assembly ® Plate for GeneAmp 9700 with dual 96-well sample block module Figure 1 Components of the 0.2-mL 96-Well Temperature Verification Kit ®...
  • Page 12 Biosystems immediately. See “Damages, claims, and returns” on page 134. Recalibration Applied Biosystems recommends that your Temperature Verification System be recalibrated once a year. For instructions on having your system recalibrated by the manufacturer, refer to Appendix A, “Recalibration.” ®...

  • Page 13: Probe Assembly

    Chapter 1 Probe assembly Probe assembly The probe assembly, shown below, consists of a cone that measures the temperature of the sample well. The temperature is measured by a thermistor bead in the cone tip. Digital thermometer Overview The handheld digital thermometer, shown below, has a temperature range of 0 to 100 °C and is accurate to within ±0.1 °C.
  • Page 14 The sample temperature that is displayed by the thermal cycler is calculated based on the ramp rate and reaction volume. Using the probe Applied Biosystems recommends that you use the probe assembly and digital while running thermometer only for the temperature verification test and the temperature non- programs uniformity test, which are described in this manual.

  • Page 15: Temperature Verification Tests For The 2720 Thermal Cycler

    Temperature Verification Tests for the 2720 Thermal Cycler This chapter covers: Overview ............8 Temperature calibration verification test.

  • Page 16: Overview

    Chapter 2 Overview Overview Materials When you perform a temperature verification test on a 2720 thermal cycler, use the materials in the kit (PN 4317939). Assembly Figure 3 shows a properly assembled system. Note that the frame is positioned around the wells with the channel facing you. The location tab on the frame should align with the locator tab on the thermal cycler.

  • Page 17: Temperature Calibration Verification Test

    Chapter 2 Temperature calibration verification test Temperature calibration verification test Use this test to verify the temperature calibration of your 2720 thermal cycler that has a 0.2-mL sample block module. The temperature calibration verification test consists of the following procedures, which you must perform in sequence: Setting up the 0.2-mL probe assembly .
  • Page 18 Chapter 2 Temperature calibration verification test 3. Press F1 (Diag). The Diagnostics screen opens. 4. Press F3 (TmpVer). The Temperature Verification screen opens. Temperature Verification Temp - Calibration Verification TNU - Temperature Non-Uniformity Temp Exit 5. Press F1 (Temp). The thermal cycler is automatically configured for the calibration verification test and the Calibration Verification screen opens.
  • Page 19 Chapter 2 Temperature calibration verification test 2. When the “Stabilizing at setpoint” value decreases to 0, read the digital thermometer. Refer to the instructions in your Temperature Verification Kit for details on operating the digital thermometer, Model 4500. 3. Using the numeric keys, enter the value that is displayed on the digital thermometer in the “Enter actual block temperature”...
  • Page 20 • If the test fails, repeat the procedure to make sure that the thermometer was not misread or that data-entry errors were not made. • If the test fails again, contact Applied Biosystems Technical Support. See “How to obtain support” on page viii.

  • Page 21: Temperature Non-Uniformity Test

    Chapter 2 Temperature non-uniformity test Temperature non-uniformity test Use this test to verify the temperature uniformity of the 2720 thermal cycler with a 0.2-mL sample block module. The temperature non-uniformity test consists of the following procedures, which you must perform in sequence: Setting up the 0.2-mL probe assembly .
  • Page 22 Chapter 2 Temperature non-uniformity test Configuring the 1. Power on the thermal cycler. The Main menu opens. For best results, allow the thermal cycler 15 to 30 minutes to warm up to ambient temperature before 2720 thermal performing the test. cycler 2.
  • Page 23 Chapter 2 Temperature non-uniformity test 2. When the sample block reaches 94 °C, the TNU Performance screen counts down the time until the setpoint is stable. TNU Performance Sample temp = xx.x°C Cover temp = xxx°C Stabilizing at setpoint x:xx Cancel 3.
  • Page 24 • If the test fails, repeat the procedure to make sure that the thermometer was not misread or that data-entry errors were not made. • If the test fails again, contact Applied Biosystems Technical Support. See “How to obtain support” on page viii.
  • Page 25 Chapter 2 Temperature non-uniformity test Ending the test 1. Press F5 (Cancel). 2. Remove the 0.2-mL probe assembly from the sample block. 3. Power off the digital thermometer, then clean off the oil. 4. Wait for the sample block to reach room temperature (~25 °C), then remove the 2720 temperature verification frame from the sample block.

  • Page 26: Data Sheet: Calibration Verification Test

    Chapter 2 Data Sheet: Calibration Verification Test Data Sheet: Calibration Verification Test When you run the calibration verification test, record the setpoint values for well A6 on this data sheet. At the end of the test, compare the values that are displayed on the thermal cycler with the values that are recorded here.

  • Page 27: Data Sheet: Temperature Non-Uniformity Test

    Chapter 2 Data Sheet: Temperature Non-Uniformity Test Data Sheet: Temperature Non-Uniformity Test When you run the temperature non-uniformity test, record the setpoint values for the wells that are listed on this data sheet. At the end of the test, compare the values that are displayed on the thermal cycler with the values that are recorded here.
  • Page 28 Chapter 2 Data Sheet: Temperature Non-Uniformity Test ® Thermal Cycler Temperature Verification System For GeneAmp PCR Systems 2720, 9600, 9700, 9800, ® and Veriti System User Guide...

  • Page 29: Temperature Verification Tests For The Geneamp 9700 Thermal Cycler

    Temperature Verification Tests for the ® GeneAmp 9700 Thermal Cycler This chapter covers: Overview ............22 Temperature calibration verification test.

  • Page 30: Overview

    Chapter 3 Overview Overview ® Materials When you perform a temperature verification test on a GeneAmp 9700 with a 96- Well Sample Block Module, use the materials in the kit (PN 4317939). Assembly Figure 5 shows a properly assembled system. Note that the frame is positioned around the wells with the channel facing you.

  • Page 31: Temperature Calibration Verification Test

    Chapter 3 Temperature calibration verification test Temperature calibration verification test ® Use this test to verify the temperature calibration of your GeneAmp 9700 thermal cycler that has a 0.2-mL sample block module. The test consists of the following procedures, which you must perform in sequence: Setting up the 0.2-mL probe assembly .
  • Page 32 Chapter 3 Temperature calibration verification test 3. Press F1 (Diag). The Diagnostics screen opens. 4. Press F3 (TmpVer). The Temperature Verification screen opens. Temperature Verification Temp - Calibration Verification TNU - Temperature Non-Uniformity Temp Exit 5. Press F1 (Temp). The thermal cycler is automatically configured for the calibration verification test and the Calibration Verification screen opens.
  • Page 33 Chapter 3 Temperature calibration verification test 2. When the “Stabilizing at setpoint” value decreases to 0, read the digital thermometer. Refer to the instructions in your Temperature Verification Kit for details on operating the digital thermometer, Model 4500. 3. Using the numeric keys, enter the value that is displayed on the digital thermometer in the “Enter actual block temperature”...
  • Page 34 • If the test fails, repeat the procedure to make sure that the thermometer was not misread or that data-entry errors were not made. • If the test fails again, contact Applied Biosystems Technical Support. See “How to obtain support” on page viii.

  • Page 35: Temperature Non-Uniformity Test

    Chapter 3 Temperature non-uniformity test Temperature non-uniformity test Use this test to verify the temperature uniformity of the GeneAmp 9700 thermal cycler with a 0.2-mL sample block module. The test consists of the following procedures, which you must perform in sequence: Setting up the 0.2-mL probe assembly .
  • Page 36 Chapter 3 Temperature non-uniformity test Configuring the 1. Power on the thermal cycler. The Main menu opens. For best results, allow the ® thermal cycler 15 to 30 minutes to warm up to ambient temperature before GeneAmp 9700 performing the test. 2.
  • Page 37 Chapter 3 Temperature non-uniformity test 2. When the sample block reaches 94 °C, the TNU Performance screen counts down the time until the setpoint is stable. TNU Performance Sample temp = xx.x°C Cover temp = xxx°C Stabilizing at setpoint x:xx Cancel 3.
  • Page 38 • If the test fails, repeat the procedure to make sure that the thermometer was not misread or that data-entry errors were not made. • If the test fails again, contact Applied Biosystems Technical Support. See “How to obtain support” on page viii.
  • Page 39 Chapter 3 Temperature non-uniformity test Ending the test 1. Press F5 (Cancel). 2. Remove the 0.2-mL probe assembly from the sample block. 3. Power off the digital thermometer, then clean off the oil. 4. Wait for the sample block to reach room temperature (~25 °C), then remove the 9700 temperature verification frame from the sample block.

  • Page 40: Data Sheet: Calibration Verification Test

    Chapter 3 Data Sheet: Calibration Verification Test Data Sheet: Calibration Verification Test When you run the calibration verification test, record the setpoint values for well A6 on this data sheet. At the end of the test, compare the values that are displayed on the thermal cycler with the values that are recorded here.

  • Page 41: Data Sheet: Temperature Non-Uniformity Test

    Chapter 3 Data Sheet: Temperature Non-Uniformity Test Data Sheet: Temperature Non-Uniformity Test When you run the temperature non-uniformity test, record the setpoint values for the wells that are listed on this data sheet. At the end of the test, compare the values that are displayed on the thermal cycler with the values that are recorded here.
  • Page 42 Chapter 3 Data Sheet: Temperature Non-Uniformity Test ® Thermal Cycler Temperature Verification System For GeneAmp PCR Systems 2720, 9600, 9700, 9800, ® and Veriti System User Guide...
  • Page 43 Temperature Verification Tests for the ® GeneAmp 9700, Dual 96-Well Module This chapter covers: Overview ............36 Calibration verification test.

  • Page 44: Overview

    Chapter 4 Overview Overview ® Materials When you perform the temperature verification test on a GeneAmp 9700 with a dual 96-well sample block module, you use the materials in the kit (PN 4317939). Assembly Figure 7 show the right frame of a properly assembled temperature verification system.

  • Page 45: Calibration Verification Test

    Chapter 4 Calibration verification test Calibration verification test ® Use this test to verify the temperature calibration of a GeneAmp 9700 with a dual 96-well sample block module. The calibration verification test consists of the following procedures, which you must perform in sequence: Setting up the 0.2-mL probe assembly .
  • Page 46 Chapter 4 Calibration verification test ® 7. For GeneAmp 9700 with Manual Dual 96-Well Sample Block Module: Slide the heated cover forward then pull the lever down. ® For GeneAmp 9700 with Auto-Lid Dual 96-Well Sample Block Module: Press the red button to slide and close the heated cover. Seat the probe properly and close the heated cover carefully.
  • Page 47 Chapter 4 Calibration verification test Running the test In this test, you take temperature readings of the sample well at two different set points using the 0.2-mL probe assembly. Note: Press F5 (Cancel) if you want to exit the test. To run the calibration verification test: 1.
  • Page 48 Chapter 4 Calibration verification test ® 4. Press Enter. The GeneAmp 9700 automatically begins the second reading (45 °C setpoint), and the Calibration Verification screen opens with the setpoint value displayed. Calibration Verification Block temp = xx.x°C Cover temp = xxx°C Setpoint is 45°C Cover must be within 1°C of 105°C Cancel...
  • Page 49 • If the test fails, repeat the procedure to make sure that the thermometer was not misread or that data-entry errors were not made. • If the test fails again, contact Applied Biosystems Technical Support. See “How to obtain support” on page viii.

  • Page 50: Temperature Non-Uniformity Test

    Chapter 4 Temperature non-uniformity test Temperature non-uniformity test ® Use this test to verify the temperature uniformity of the GeneAmp 9700 with Dual 96-Well Sample Block Module. The temperature non-uniformity test consists of the following procedures, which you must perform in sequence: Setting up the 0.2-mL probe assembly.
  • Page 51 Chapter 4 Temperature non-uniformity test ® 7. For the GeneAmp 9700 with Manual Dual 96-Well Sample Block Module: Slide the heated cover forward and pull the lever down. ® For the GeneAmp 9700 with Auto-Lid Dual 96-Well Sample Block Module: Press the red button to slide and close the heated cover.
  • Page 52 Chapter 4 Temperature non-uniformity test Running the test The temperature non-uniformity test uses the 0.2-mL probe assembly to test the temperature uniformity of 6 different wells in each sample block. Note: Press F5 (Cancel) if you want to exit the test. To run the temperature non-uniformity test: 1.
  • Page 53 Chapter 4 Temperature non-uniformity test ® 4. Press Enter. The GeneAmp 9700 automatically begins the second reading (55 °C setpoint), and the TNU Performance screen opens with the setpoint value displayed. TNU Performance Sample temp = xx.x°C Cover temp = xxx°C Setpoint is 55°C Sample must be within 1.0°C of setpoint Cancel...
  • Page 54 • If the test fails, repeat the procedure to make sure that the thermometer was not misread or that data-entry errors were not made. • If the test fails again, contact Applied Biosystems Technical Support. See “How to obtain support” on page viii.

  • Page 55: Data Sheet: Calibration Verification Test

    Chapter 4 Data Sheet: Calibration Verification Test Data Sheet: Calibration Verification Test When you run the calibration verification test, record the setpoint values for well R3 on this data sheet. At the end of the test, compare the values that are displayed on the thermal cycler with the values that are recorded here.

  • Page 56: Thermal Cycler Temperature Verification System For Geneamp ® Pcr Systems 2720, 9600, 9700, 9800

    Chapter 4 Data Sheet: Temperature Non-Uniformity Test Data Sheet: Temperature Non-Uniformity Test When you run the temperature non-uniformity test, record the setpoint values for the wells L1 to L6 and R1 to R6 listed on this data sheet. At the end of the test, compare the values that are displayed on the thermal cycler with the values that are recorded here.
  • Page 57 ® Temperature Verification Tests for the GeneAmp 9700, Dual 384-Well Sample Block Module This chapter covers: Calibration verification test..........50 Temperature non-uniformity test .

  • Page 58: Calibration Verification Test

    Chapter 5 Calibration verification test Calibration verification test Use the calibration verification test to verify the temperature calibration of your ® PCR System 9700 Sample Block Module The calibration GeneAmp , Dual 384-Well verification test consists of the following procedures, which you must perform in sequence: Setting up the 0.02-mL probe assembly.
  • Page 59 Chapter 5 Calibration verification test 5. Thread the probe wire through the channels in the 384-well temperature verification plate to prevent damage to the probe and lead wires. 6. Make sure the probe is connected to the digital thermometer. 7. Slide the heated cover forward and pull the lever down. Seat the probe properly, and close the heated cover carefully.
  • Page 60 Chapter 5 Calibration verification test To run the calibration verification test: ® 1. Complete the procedures in “Configuring the GeneAmp 9700” on page 2. Press F1 (Run) to start the calibration verification test. The Calibration Verification screen opens with the setpoint value displayed. Calibration Verification Block temp = xx.x°C Cover temp = xxx°C Setpoint is 85°C...
  • Page 61 Chapter 5 Calibration verification test ® 5. Press ENTER. The GeneAmp 9700 automatically begins the second reading (45 °C setpoint). The Calibration Verification screen opens with the setpoint value displayed. Calibration Verification Block temp = xx.x°C Cover temp = xxx°C Setpoint is 45°C Cover must be within 1°C of 105°C Cancel...
  • Page 62 • If the test fails, repeat the procedure to make sure that the thermometer was not misread or that data-entry errors were not made. • If the test fails again, contact Applied Biosystems Technical Support. See “How to obtain support” on page viii.

  • Page 63: Temperature Non-Uniformity Test

    Chapter 5 Temperature non-uniformity test Temperature non-uniformity test ® Use this test to verify the temperature uniformity of the GeneAmp PCR System 9700, Dual 384-Well Sample Block Module. The temperature non-uniformity test consists of the following procedures, which you must perform in sequence: Setting up the 0.02-mL probe assembly .
  • Page 64 Chapter 5 Temperature non-uniformity test 5. Thread the probe wire through the channels in the 384-well temperature verification plate to prevent damage to the probe and lead wires. 6. Make sure the probe is connected to the digital thermometer. 7. Slide the heated cover forward and pull the lever down. Seat the probe properly and close the heated cover carefully.
  • Page 65 Chapter 5 Temperature non-uniformity test Running the test The temperature non-uniformity test uses the 0.02-mL probe assembly to test the temperature uniformity of six different wells in each sample block. If necessary, press F5 (Cancel) to exit the test. Note: To run the temperature non-uniformity test: 1.
  • Page 66 Chapter 5 Temperature non-uniformity test ® 5. Press ENTER. The GeneAmp 9700 automatically begins the second reading (37 °C setpoint). The TNU Performance screen opens with the setpoint value displayed. TNU Performance Sample temp = xx.x°C Cover temp = xxx°C Setpoint is 37°C Sample must be within 1.0°C of setpoint Cancel...
  • Page 67 • If the test fails, repeat the procedure to make sure that the thermometer was not misread or that data-entry errors were not made. • If the test fails again, contact Applied Biosystems Technical Support. See “How to obtain support” on page viii.

  • Page 68: Data Sheet: Calibration Verification Test

    Chapter 5 Data Sheet: Calibration Verification Test Data Sheet: Calibration Verification Test When running the calibration verification test, record the setpoint values for well R3 on this data sheet. At the end of the test, compare the values that are displayed on the thermal cycler with the values that are recorded here.

  • Page 69: Data Sheet: Temperature Non-Uniformity Test

    Chapter 5 Data Sheet: Temperature Non-Uniformity Test Data Sheet: Temperature Non-Uniformity Test When running the temperature non-uniformity test, record the setpoint values for the wells listed on this data sheet. At the end of the test, compare the values that are displayed on the thermal cycler with the values that are recorded here.
  • Page 70 Chapter 5 Data Sheet: Temperature Non-Uniformity Test ® Thermal Cycler Temperature Verification System For GeneAmp PCR Systems 2720, 9600, 9700, 9800, ® and Veriti System User Guide...
  • Page 71 ® Temperature Verification Tests for the GeneAmp 9700, Auto-Lid Sample Block Module This chapter covers: Calibration verification test ......... . . 64 Temperature non-uniformity test .

  • Page 72: Calibration Verification Test

    Chapter 6 Calibration verification test Calibration verification test Use the calibration verification test to verify the temperature calibration of your ® GeneAmp PCR System 9700, Auto-Lid Sample Block Module. The verification test consists of the following procedures, which you must perform in sequence: Setting up the 20-µL probe assembly.
  • Page 73 Chapter 6 Calibration verification test 3. Use the stick end of a cotton swab to coat well R3 with mineral oil; The wells are too small for the swab end. 4. Place the 20-µL probe assembly into well R3. 5. Thread the probe wire through the channels in the 384-well temperature verification plate to prevent damage to the probe and lead wires.
  • Page 74 Chapter 6 Calibration verification test Running the test Use the thermometer to take temperature readings of the well that is connected to the 20-µL probe assembly. You must take readings at two different setpoint temperatures. If necessary, press F5 (Cancel) to exit the test. Note: To run the calibration verification test: 1.
  • Page 75 Chapter 6 Calibration verification test ® 4. Press Enter. The GeneAmp 9700 automatically begins the second reading (45 °C setpoint). The Calibration Verification screen opens with the setpoint value displayed. Calibration Verification Block temp = xx.x°C Cover temp = xxx°C Setpoint is 45°C Cover must be within 1°C of 105°C Cancel...
  • Page 76 • If the test fails, repeat the procedure to make sure that the thermometer was not misread or that data-entry errors were not made. • If the test fails again, contact Applied Biosystems Technical Support. See “How to obtain support” on page viii.

  • Page 77: Temperature Non-Uniformity Test

    Chapter 6 Temperature non-uniformity test Temperature non-uniformity test ® Use this test to verify the temperature uniformity of the GeneAmp PCR System 9700, Auto-Lid Sample Block Module. The temperature non-uniformity test consists of the following procedures, which you must perform in sequence: Setting up the 20-µL probe assembly .
  • Page 78 Chapter 6 Temperature non-uniformity test 3. Use the stick end of a cotton swab to coat wells L1 to L6 and R1 to R6 with mineral oil; the wells are too small for the swab end. 4. Place the 20-µL probe assembly into well L1. As the test progresses, move the 20-µL probe assembly to each of the test Note: wells (L1 to L6 and R1 to R6).
  • Page 79 Chapter 6 Temperature non-uniformity test To run the temperature non-uniformity test: 1. Press F1 (Run) to start the temperature non-uniformity test. The TNU Performance screen opens with the setpoint value displayed. TNU Performance Sample temp = xx.x°C Cover temp = xxx°C Setpoint is 94°C Sample must be within 1.0°C of setpoint Cancel...
  • Page 80 Chapter 6 Temperature non-uniformity test ® 4. Press Enter. The GeneAmp 9700 automatically begins the second reading (37 °C setpoint). The TNU Performance screen opens with the setpoint value displayed. TNU Performance Sample temp = xx.x°C Cover temp = xxx°C Setpoint is 37°C Sample must be within 1.0°C of setpoint Cancel...
  • Page 81 • If the test fails, repeat the procedure to make sure that the thermometer was not misread or that data-entry errors were not made. • If the test fails again, contact Applied Biosystems Technical Support. See “How to obtain support” on page viii.

  • Page 82: Data Sheet: Calibration Verification Test

    Chapter 6 Data Sheet: Calibration Verification Test Data Sheet: Calibration Verification Test When running the calibration verification test, record the setpoint values for well R3 on this data sheet. At the end of the test, compare the values that are displayed on the thermal cycler with the values that are recorded here.

  • Page 83: Data Sheet: Temperature Non-Uniformity Test

    Chapter 6 Data Sheet: Temperature Non-Uniformity Test Data Sheet: Temperature Non-Uniformity Test When running the temperature non-uniformity test, record the setpoint values for the wells listed on this data sheet. At the end of the test, compare the values that are displayed on the thermal cycler with the values that are recorded here.
  • Page 84 Chapter 6 Data Sheet: Temperature Non-Uniformity Test ® Thermal Cycler Temperature Verification System For GeneAmp PCR Systems 2720, 9600, 9700, 9800, ® and Veriti System User Guide...
  • Page 85 ® Temperature Verification Tests for the GeneAmp 9700, 0.5-mL Sample Block Module This chapter covers: Calibration verification test..........78 Temperature non-uniformity test .

  • Page 86: Calibration Verification Test

    Chapter 7 Calibration verification test Calibration verification test Use the calibration verification test to verify the temperature calibration of your ® PCR System 9700 Sample Block Module The calibration GeneAmp , 0.5-mL verification test consists of the following procedures, which you must perform in sequence: Setting up the 0.5-mL probe assembly.
  • Page 87 Chapter 7 Calibration verification test Configuring the 1. Complete the procedures in “Setting up the 0.5-mL probe assembly” on ® page GeneAmp 9700 2. Power on the thermal cycler. The Main menu opens. For best results, allow the thermal cycler 15 to 30 minutes to warm up to ambient temperature before performing the test.
  • Page 88 Chapter 7 Calibration verification test Running the test Use the digital thermometer to take temperature readings of the sample well that is connected to the 0.5-mL probe assembly. You must take readings at two different setpoint temperatures. If necessary, press F5 (Cancel) to exit the test. Note: To run the calibration verification test: ®...
  • Page 89 Chapter 7 Calibration verification test ® 5. Press ENTER. The GeneAmp 9700 automatically begins the second reading (45 °C setpoint). The Calibration Verification screen opens with the setpoint value displayed. Calibration Verification Block temp = xx.x°C Cover temp = xxx°C Setpoint is 45°C Cover must be within 30°C of 45°C Cancel...
  • Page 90 • If the test fails, repeat the procedure to make sure that the thermometer was not misread or that data-entry errors were not made. • If the test fails again, contact Applied Biosystems Technical Support. See “How to obtain support” on page viii.

  • Page 91: Temperature Non-Uniformity Test

    Chapter 7 Temperature non-uniformity test Temperature non-uniformity test ® Use this test to verify the temperature uniformity of the PCR System GeneAmp 9700 0.5-mL Sample Block Module. The temperature non-uniformity test consists of the following procedures, which you must perform in sequence: Setting up the 0.5-mL probe assembly .
  • Page 92 Chapter 7 Temperature non-uniformity test 3. Press F4 (Util). The Utilities screen opens. 4. Press F1 (Diag). The Diagnostics screen opens. 5. Press F3 (TmpVer). The Temperature Verification screen opens. Temperature Verification Temp - Calibration Verification TNU - Temperature Non-Uniformity Temp Exit ®...
  • Page 93 Chapter 7 Temperature non-uniformity test 3. The TNU Performance screen counts down the time until the setpoint is stable. When the “Stabilizing at setpoint” value decreases to 0, read the thermometer. TNU Performance Sample temp = xx.x°C Cover temp = xxx°C Stabilizing at setpoint...
  • Page 94 Chapter 7 Temperature non-uniformity test 8. Slide the heated cover back, and repeat steps 4 through 7 “Setting up the 0.5- mL probe assembly” on page 83 steps 2 through 7 of this procedure. Complete these steps for all 12 wells to be tested: A1, A6, A10, B3, C5, C10, D1, D7, E8, F1, F5, and F10 ®...
  • Page 95 • If the test fails, repeat the procedure to make sure that the thermometer was not misread or that data-entry errors were not made. • If the test fails again, contact Applied Biosystems Technical Support. See “How to obtain support” on page viii.

  • Page 96: Data Sheet: Calibration Verification Test

    Chapter 7 Data Sheet: Calibration Verification Test Data Sheet: Calibration Verification Test When running the calibration verification test, record the setpoint values for well A6 on this data sheet. At the end of the test, compare the values that are displayed on the thermal cycler with the values that are recorded here.

  • Page 97: Data Sheet: Temperature Non-Uniformity Test

    Chapter 7 Data Sheet: Temperature Non-Uniformity Test Data Sheet: Temperature Non-Uniformity Test When running the temperature non-uniformity test, record the setpoint values for the wells listed on this data sheet. At the end of the test, compare the values that are displayed on the thermal cycler with the values that are recorded here.
  • Page 98 Chapter 7 Data Sheet: Temperature Non-Uniformity Test ® Thermal Cycler Temperature Verification System For GeneAmp PCR Systems 2720, 9600, 9700, 9800, ® and Veriti System User Guide...
  • Page 99 Temperature Verification Tests for the ® GeneAmp 9800 This chapter covers: Calibration verification test..........92 Temperature non-uniformity test .

  • Page 100: Calibration Verification Test

    Calibration verification test Use the calibration verification test to verify the temperature calibration of your Applied Biosystems GenAmp 9800 Fast Thermal Cycler with 96-Well Aluminum Sample Block Module. The calibration verification test consists of the following procedures, which you must perform in sequence: Setting up the 0.1-mL probe...
  • Page 101 Chapter 8 Calibration verification test Configuring the 1. Complete the procedures in “Setting up the 0.1-mL probe assembly” on ® page GeneAmp 9800 ® 2. Power on the GeneAmp 9800. For best results, allow the thermal cycler 15 to 30 minutes to warm up to ambient temperature before performing the test.
  • Page 102 Chapter 8 Calibration verification test 2. Press F1 (Run) to start the calibration verification test. The Calibration Verification screen opens with the setpoint value displayed. Calibration Verification Block temp = xx.x°C Cover temp = xxx°C Setpoint is 85°C Cover must be within 10°C of 85°C Cancel The Calibration Verification screen counts down the time until the setpoint is reached.
  • Page 103 Chapter 8 Calibration verification test ® 5. Press Enter. The GeneAmp 9800 begins the second reading (45 °C setpoint), and the Calibration Verification screen opens with the setpoint value displayed. Calibration Verification Block temp = xx.x°C Cover temp = xxx°C Setpoint is 45°C Cover must be within 30°C of 45°C Cancel...
  • Page 104 • If the test fails, repeat the procedure to make sure that the thermometer was not misread or that data-entry errors were not made. • If the test fails again, contact Applied Biosystems Technical Support. See “How to obtain support” on page viii.

  • Page 105: Temperature Non-Uniformity Test

    Chapter 8 Temperature non-uniformity test Temperature non-uniformity test Use this test to verify the temperature uniformity of the Applied Biosystems ® GeneAmp 9800 Fast Thermal Cycler with 96-Well Aluminum Sample Block Module. The temperature non-uniformity test consists of the following procedures, which you must perform in sequence: Setting up the 0.1-mL probe assembly...
  • Page 106 Chapter 8 Temperature non-uniformity test Configuring the 1. Complete the procedures in “Setting up the 0.1-mL probe assembly” on ® page GeneAmp 9800 ® 2. Power on the GeneAmp 9800. For best results, allow the thermal cycler 15 to 30 minutes to warm up to ambient temperature before performing the test.
  • Page 107 Chapter 8 Temperature non-uniformity test Running the test The temperature non-uniformity test uses the 0.1-mL probe assembly to test the temperature uniformity of six different wells in each sample block. Note: If necessary, press F5 (Cancel) to exit the test. To run the temperature non-uniformity test: 1.
  • Page 108 Chapter 8 Temperature non-uniformity test 5. Using the numeric keys, enter the value that is displayed on the digital thermometer into the “Enter actual block temperature” field. TNU Performance Sample temp = xx.x°C Cover temp = xxx°C Enter actual block temperature 00.0 Cancel Round the four-digit value on the digital thermometer to three digits Note:...
  • Page 109 Chapter 8 Temperature non-uniformity test 8. Slide the heated cover back, and repeat steps 4 through 8 “Setting up the 0.1- mL probe assembly” on page 97 steps 2 through 7 of this procedure at 50 °C. Complete these steps for all eight wells to be tested: A1, A12, C4, C9, F4,F9, H1, and H12 The following screen opens: TNU Performance...
  • Page 110 • If the test fails, repeat the procedure to make sure that the thermometer was not misread or that data-entry errors were not made. • If the test fails again, contact Applied Biosystems Technical Support. See “How to obtain support” on page viii.

  • Page 111: Data Sheet: Calibration Verification Test

    Chapter 8 Data Sheet: Calibration Verification Test Data Sheet: Calibration Verification Test When running the calibration verification test, record the setpoint values for well A6 on this data sheet. At the end of the test, compare the values that are displayed on the thermal cycler with the values that are recorded here.

  • Page 112: Data Sheet: Temperature Non-Uniformity Test

    Chapter 8 Data Sheet: Temperature Non-Uniformity Test Data Sheet: Temperature Non-Uniformity Test When running the temperature non-uniformity test, record the setpoint values for wells A1, A12, C4, C9, F4, F9, H1, and H12 on this data sheet. At the end of the test, compare the values that are displayed on the thermal cycler with the values that are recorded here.
  • Page 113 Temperature Verification Tests for the ® GeneAmp 9600 This chapter covers: Overview ............106 Calibration verification test.

  • Page 114: Overview

    Chapter 9 Overview Overview ® Materials When you perform temperature verification tests on the GeneAmp 9600, you use the materials provided in your kit. Assembly Figure 18 shows a properly assembled system. The frame is positioned around the wells with the channel facing you, and the probe wire is threaded through the channel to prevent damaging the wire when the thermal cycler heated cover is closed.

  • Page 115: Calibration Verification Test

    Chapter 9 Calibration verification test Calibration verification test ® Use this test to verify the temperature calibration of your GeneAmp 9600. The calibration verification test consists of the following procedures, which you must perform in sequence: Setting up the 0.2-mL probe assembly .
  • Page 116 Chapter 9 Calibration verification test ® Running the test 1. Power on the GeneAmp 9600. The Main menu opens. For best results, allow the thermal cycler 15 to 30 minutes to warm up to ambient temperature before performing the test. 2.
  • Page 117 Chapter 9 Calibration verification test 6. Press ENTER. The temperature of the sample block and heated cover go to 95 °C, and the following display opens: Going to 95°C... Cvr = xxC Blk = xx.xC This display shows the current temperature of the block cover (Cvr = xxC) and sample block (Blk = xx.xC).
  • Page 118 Chapter 9 Calibration verification test Average block temperature at the 95 °C hold Use the following formula to calculate the average block temperature at the 95 °C hold: Block Average at 95 °C = T(95) – High Offset If the block average is more than 0.75 degrees Celsius above or below 95 °C, your ®...

  • Page 119: Temperature Non-Uniformity Test

    Chapter 9 Temperature non-uniformity test Temperature non-uniformity test ® Use this test to verify the temperature uniformity of the GeneAmp 9600. The temperature non-uniformity test consists of the following procedures, which you must perform in sequence: Setting up the 0.2-mL probe assembly .
  • Page 120 Chapter 9 Temperature non-uniformity test 2. Set up a two-temperature CYCL program with the following parameters: • Setpoint #1 Temperature = 95 °C • Hold Time = 2:00 minutes • Ramp Time = 0:00 minutes • Setpoint #2 Temperature = 40 °C •...
  • Page 121 If either result is more than 1 °C from the setpoint, your GeneAmp 9600 must be serviced by an Applied Biosystems service representative. Ending the test 1. Remove the 0.2-mL probe assembly from the sample block. 2. Power off the digital thermometer, then clean off the oil.

  • Page 122: Data Sheet: Calibration Verification Test

    Chapter 9 Data Sheet: Calibration Verification Test Data Sheet: Calibration Verification Test When you run the calibration verification test, record the setpoint values for well E1 on this data sheet. At the end of the test, compare the values that are displayed on the thermal cycler with the values that are recorded here.

  • Page 123: Data Sheet: Temperature Non-Uniformity Test

    Chapter 9 Data Sheet: Temperature Non-Uniformity Test Data Sheet: Temperature Non-Uniformity Test When you run the temperature non-uniformity test, record the setpoint values for the wells listed on this data sheet. At the end of the test, compare the values that are displayed on the thermal cycler with the values that are recorded here.
  • Page 124 Chapter 9 Data Sheet: Temperature Non-Uniformity Test ® Thermal Cycler Temperature Verification System For GeneAmp PCR Systems 2720, 9600, 9700, 9800, ® and Veriti System User Guide...
  • Page 125 Temperature Verification Tests for the ® Veriti Thermal Cycler This chapter covers: Overview ............118 Calibration verification test.

  • Page 126: Overview

    Chapter 10 Overview Overview ® Materials When you perform temperature verification tests on the Veriti Thermal Cycler, you use the materials provided in your kit. Assembly Figure 19 shows a properly assembled system. The frame is positioned around the wells with the channel facing you, and the probe wire is threaded through the channel to prevent damaging the wire when the thermal cycler heated cover is closed.

  • Page 127: Calibration Verification Test

    Chapter 10 Calibration verification test Calibration verification test ® Use this test to verify the temperature calibration of your Veriti Thermal Cycler. The calibration verification test consists of the following procedures, which you must perform in sequence: Setting up the 0.5-mL probe assembly .
  • Page 128 Chapter 10 Calibration verification test 5. Place the 0.5-mL Thermal Isolation Frame onto the sample block. 6. Using a cotton swab, coat the following wells with mineral oil: B3, B8, F1, F10 7. Place the 0.5-mL probe assembly into well B3. Thread the probe assembly wire through the channel in the temperature verification frame to prevent damage to the probe assembly and lead wires (see Figure 19 on page...
  • Page 129 Chapter 10 Calibration verification test Running the test 1. In the Tools Menu, select Run Temperature Verification. 2. Press → to begin the test. Perform the test as directed by the touchscreen. As the test progresses, move the 0.5-mL probe assembly to the remaining test wells: B8, F1, F10 3.
  • Page 130 Chapter 10 Calibration verification test Ending the test 1. If the heated cover is closed, lift the lever and place the heated cover in the fully opened position. PHYSICAL INJURY HAZARD. Hot Surface. Use care when working around the heated cover and sample block to avoid being burned by hot components.

  • Page 131: Temperature Non-Uniformity Test

    Chapter 10 Temperature non-uniformity test Temperature non-uniformity test ® Use this test to verify the temperature uniformity of the Veriti Thermal Cycler. The temperature non-uniformity test consists of the following procedures, which you must perform in sequence: Setting up the 0.5-mL probe assembly .
  • Page 132 Chapter 10 Temperature non-uniformity test 5. Place the 0.5-mL Thermal Isolation Frame onto the sample block. 6. Using a cotton swab, coat the following wells with mineral oil: A1, A10, B3, B8, E3, E8, F1, F10 7. Place the 0.5-mL probe assembly into well A1. Thread the probe wire through the channel in the temperature verification frame to prevent damage to the probe assembly and lead wires (see Figure 19 on page...
  • Page 133 Chapter 10 Temperature non-uniformity test Running the test 1. In the Tools menu, select Run TNU Test. 2. Press → to begin the test. Perform the test as directed by the touchscreen. As the test progresses, move the 0.5-mL probe assembly to the remaining test wells: A1, A10, B3, B8, E3, E8, F1, F10 3.
  • Page 134 Chapter 10 Temperature non-uniformity test Ending the test 1. If the heated cover is closed, lift the lever and place the heated cover in the fully opened position. PHYSICAL INJURY HAZARD. Hot Surface. Use care when working around the heated cover and sample block to avoid being burned by hot components.

  • Page 135: Data Sheet: Calibration Verification Test

    Chapter 10 Data Sheet: Calibration Verification Test Data Sheet: Calibration Verification Test When you run the calibration verification test, record the setpoint values for well E1 on this data sheet. At the end of the test, compare the values that are displayed on the thermal cycler with the values that are recorded here.

  • Page 136: Data Sheet: Temperature Non-Uniformity Test

    Chapter 10 Data Sheet: Temperature Non-Uniformity Test Data Sheet: Temperature Non-Uniformity Test When you run the temperature non-uniformity test, record the setpoint values for the wells listed on this data sheet. At the end of the test, compare the values that are displayed on the thermal cycler with the values that are recorded here.
  • Page 137 This appendix describes how to order a recalibration service for your Temperature Verification System. Obtaining recalibration Introduction Applied Biosystems recommends that the Model 4500 digital thermometer and Probe Assembly be recalibrated on an annual basis. To have your thermometer and probe recalibrated: 1. Decontaminate the probe assembly (see below).
  • Page 138 Appendix A 4. Create a package that contains the: • Black case containing digital thermometer and probe assembly • Address and contact information: • Billing address • Shipping address • Name and phone number of a contact; preferably the person most familiar with the thermometer 5.
  • Page 139 Instrument Warranty Information This chapter covers: Limited product warranty ..........132 Damages, claims, and returns .
  • Page 140 Applied Biosystems warrants that for a period of ninety (90) days from the date the warranty period begins, the tapes, diskettes, or other media bearing the operating software of the product, if any, will be free of defects in materials and workmanship under normal use.
  • Page 141 Applied Biosystems; and modification or repair of the product not authorized by Applied Biosystems.
  • Page 142 Returns Do not return any material without prior notification and authorization. If for any reason it becomes necessary to return material to Applied Biosystems, contact Applied Biosystems Technical Support or your nearest Applied Biosystems subsidiary or distributor for a return authorization (RA) number and forwarding address.
  • Page 143 • The TVK has accuracy and discrepancy in addition to the original manufacturing specification. • Temperature calibration of Applied Biosystems thermal cyclers is extremely stable, and no measurable change in the calibration accuracy is detectable unless a significant change occurs in the instrument characteristics after its manufacture.
  • Page 144 Appendix C ® Thermal Cycler Temperature Verification System For GeneAmp PCR Systems 2720, 9600, 9700, 9800, ® and Veriti System User Guide...
  • Page 145 Safety This appendix covers: Instrumentation safety ..........138 Symbols on instruments .
  • Page 146 Instrumentation safety Symbols on instruments Electrical The following table describes the electrical symbols that may be displayed on symbols on Applied Biosystems instruments. instruments Symbol Description Indicates the On position of the main power switch. Indicates the Off position of the main power switch.
  • Page 147 Safety symbols The following table describes the safety symbols that may be displayed on Applied Biosystems instruments. Each symbol may appear by itself or with text that explains the relevant hazard. These safety symbols may also appear next to DANGERS, WARNINGS, and CAUTIONS that occur in the text of this and other product-support documents.
  • Page 148 Appendix D Safety ® Thermal Cycler Temperature Verification System For GeneAmp PCR Systems 2720, 9600, 9700, 9800, ® and Veriti System User Guide...
  • Page 149 Index Calibration Verification Test equipment overview – system 2720 – system 9600 – system 9700 – hazard icons. See safety symbols, on instruments system 9700 with 0.5 mL sample block module system 9700 with Auto-Lid sample block hazard symbols. See safety symbols, on instruments –...
  • Page 150 Temperature Non-Uniformity Test – system 2720 – system 9600 – system 9700 – system 9700 with 0.5 mL sample block module system 9700 with Auto-Lid sample block – module system 9700 with dual 384-well sample block – module system 9700 with dual 96-well sample block –...
  • Page 151 150 countries on six continents. For sales office locations and technical support, please call our local office or refer to our Web site at www.appliedbiosystems.com. Applied Biosystems is committed to providing the world’s leading technology and information for life scientists. Headquarters...

This manual is also suitable for:

Geneamp 9600Geneamp 9700Geneamp 9800

Table of Contents