Applied Biosystems 7900HT User Manual page 247

Cleaning a
Sample Block
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
This procedure applies to a:
• Standard 384-Well Block
• Standard 96-Well Block
• Fast 96-Well Block
1. Remove the sample block from the 7900HT instrument as explained in
"Removing the Sample Block" on page
2. Use the following figure as a guide to locate the suspected contaminated wells
on the sample block.
3. Pipette the appropriate volume of 10% bleach solution into each suspected
contaminated well of the sample block module.
• For a Standard 384-Well Block, pipette 40 µL bleach solution to each well.
• For a Standard 96-Well Block, pipette 150 µL bleach solution to each well.
• For a Fast 96-Well Block, pipette 55 µL bleach solution to each well.
4. Allow the sample block to sit for 3 to 5 min.
5. Using a pipet, remove the bleach solution from the wells of the sample block.
6. Rinse (pipette and remove) each contaminated well with three treatments of
deionized water at the appropriate volume for the sample block.
• For a Standard 384-Well Block, rinse affected wells with 40 µL deionized
water.
• For a Standard 96-Well Block, rinse affected wells with 150 µL deionized
water.
• For a Fast 96-Well Block, rinse affected wells with 55 µL deionized water.
Absolute isopropanol can be substituted for water in the third treatment.
Note:
7. Remove any remaining isopropanol or water from the wells of the sample block
module.
8. Replace the sample block as explained in
page 7-10.
9. Run a background plate to confirm that the contamination has been removed.
7-7.
Circuitry and connections to
the instrument (Do Not Touch)
Warning
Well A-1
"Replacing the Sample Block" on
DRAFT
September 1, 2004 11:39 am, CH_Maintenance.fm
Decontaminating the Sample Block
7-15