Lesson 1: Using Plate Documents - Applied Biosystems 7900HT User Manual

Chapter 2 Getting Started

Lesson 1: Using Plate Documents

About SDS Plate
Documents
2-12 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:38 am, CH_GetStart.fm
Every plate run on the Applied Biosystems 7900HT Fast Real-Time PCR System
requires the creation of a plate document using the SDS software. A plate document
is a virtual representation of a consumable used to contain samples and reagents
during a sequence detection run. The software uses the plate document to coordinate
the operation of the instrument (thermal cycling and data collection), to organize and
store the data gathered during the PCR, and to analyze the run data.
Table 2-3 Plate Document Types of the SDS Software
File Ext.
SDS 7900HT *.sds
Document
SDS 7900HT *.sdt
Template
Document
SDS 7900HT *.sdm
Multiple Plate
Document
The exercises in this lesson will familiarize you with the use of plate documents.
DRAFT
Icon Description
Plate Documents are the primary file you will use.
They are generated for every kind of experiment
and are generally used to run plates.
Although optional, plate document templates are
useful as timesaving devices for experiments where
samples are run on plates with identical assay
configurations.
Multiple plate documents are used to analyze data
from relative quantification experiments. The use of
multiple plate documents is discussed in detail in
Chapter 6, "Analyzing Real-Time Data."