Applied Biosystems 7900HT User Manual page 131

Placing the
Buckets in the
Centrifuge
Centrifuging
the Cards
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
1. Make sure the centrifuge is powered on, but is not rotating.
2. Open the centrifuge cover by pressing the OPEN button.
3. Place a loaded bucket onto an open rotor arm of the centrifuge.
Make sure the bucket can swing easily within its slotted position on the
Note:
rotor arm.
4. Place the remaining buckets onto the rotor arms, per step 3.
The manufacturer recommends running the centrifuge with
all four buckets, even if only two buckets contain Low Density Arrays.
Make sure the buckets and their contents are balanced.
Opposing buckets should have matching weights. If the buckets are not fully
loaded with Low Density Arrays containing the sample-specific PCR reaction
mix, place blank balance cards and card holders into the buckets.
5. Close the centrifuge cover.
1. Press the START button.
START
button
The centrifuge starts, then automatically stops after 1 min, per the programmed
sequence.
2. Repeat step 1 so that the Low Density Arrays are centrifuged for a total of two
consecutive, 1-min spins to ensure complete distribution of the sample-specific
PCR reaction mix.
3. Open the centrifuge cover by pressing the OPEN button.
Preparing TaqMan Low Density Arrays for Use
OPEN cover button
DRAFT
September 1, 2004 11:39 am, CH_Basic.fm
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