Applied Biosystems 7300 Installation And Maintenance Manual
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Applied Biosystems 7300 Installation And Maintenance Manual

Real-time pcr system
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Applied Biosystems 7300/7500/7500 Fast
Real-Time PCR System
Installation and
Maintenance Guide
Before Starting
the Installation
Unpacking and
Setting Up
the Instrument
Installing
the Software and
Powering On the
Instrument
Performing the
Regions of
Interest (ROI)
Calibration
Performing the
Background
Calibration and
Optical Calibration
Performing the
Pure Dye
Calibration
Verifying the
Instrument
Performance
Powering Off the
7300/7500/7500
Fast System

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Summary of Contents for Applied Biosystems 7300

  • Page 1 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Before Starting Installation and the Installation Maintenance Guide Unpacking and Setting Up the Instrument Installing the Software and Powering On the Instrument Performing the Regions of Interest (ROI) Calibration Performing the Background Calibration and...
  • Page 2 Information in this document is subject to change without notice. Applied Biosystems assumes no responsibility for any errors that may appear in this document. In no event shall Applied Biosystems be liable for incidental, special, multiple, or consequential damages in connection with or arising from the use of this document.
  • Page 3 Installing the Software and Powering On the Instrument Performing the ROI Calibration Performing the Background Calibration Performing the Chapter Pure Dye Icon Calibration Verifying the Instrument Performance Installation and Maintenance Guide for the Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System...
  • Page 4 Read This First Installation and Maintenance Guide for the Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System...

  • Page 5: 7300/7500/7500 Fast System

    RNase P Plate Data shooting Performance Performance Powering Off the Powering Off the Performing a Short-Term Performing a Long-Term Chapter 8 7300/7500/ 7300/7500/ Shutdown Shutdown 7500 Fast System 7500 Fast System Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 6 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 7: Table Of Contents

    Checking the Shipped Materials ........14 Setting Up the 7300/7500/7500 Fast Instrument ......20...
  • Page 8 Overview ............24 Installing the SDS Software for the 7300/7500/7500 Fast System ....25 Powering On the Instrument .
  • Page 9 Cleaning Up and Defragmenting the Hard Drive ......114 Moving the 7300/7500/7500 Fast System ....... 115 Monitoring Lamp Status .
  • Page 10 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 11: Preface

    • Applied Biosystems 7300/7500/7500 Fast System Setup and Maintenance Video CD provided with the instrument. This guide is intended for novice and experienced Applied Biosystems 7300, 7500, and Audience 7500 Fast Real-Time PCR System users who need to install, maintain, or troubleshoot their system.

  • Page 12: How To Obtain More Information

    “Safety Safety Alert Alert Words” on page xvi. Words How to Obtain More Information For more information about using the 7300/7500/7500 Fast system, refer to the Related documents shown below. Documentation Online Help Document Title Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System...

  • Page 13: How To Obtain Support

    • Order Applied Biosystems user documents, MSDSs, certificates of analysis, and other related documents • Download PDF documents • Obtain information about customer training • Download software updates and patches xiii Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 14 Preface How to Obtain Support Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 15 ..........xxv ■ Safety and Electromagnetic Compatibility (EMC) Standards ... . . xxv Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 16: Safety And Emc Compliance Information

    Safety Conventions Used in This Document Safety Conventions Used in This Document Four safety alert words appear in Applied Biosystems user documentation at points in Safety Alert the document where you need to be aware of relevant hazards. Each alert Words word–IMPORTANT, CAUTION, WARNING, DANGER–implies a particular level of...

  • Page 17: Symbols On Instruments

    The following table describes the safety symbols that may be displayed on Safety Symbols Applied Biosystems instruments. Each symbol may appear by itself or in combination with text that explains the relevant hazard (see “Safety Labels on Instruments”...

  • Page 18: Safety Labels On Instruments

    European Union. Safety Labels on Instruments The following CAUTION, WARNING, and DANGER statements may be displayed on Applied Biosystems instruments in combination with the safety symbols described in the preceding section. English Français CAUTION Hazardous chemicals.
  • Page 19 Safety Labels on Instruments Locations of The Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System contain warnings at the locations shown below. Warnings Attention Physical hazard Physical hazard Attention Attention Attention Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 20: General Instrument Safety

    PHYSICAL INJURY HAZARD. Use this product only as specified in this document. Using this instrument in a manner not specified by Applied Biosystems may result in personal injury or damage to the instrument. PHYSICAL INJURY HAZARD. The instrument is to be moved...

  • Page 21: Chemical Safety

    Each time you receive a new MSDS packaged with a hazardous chemical, be sure to replace the appropriate MSDS in your files. You can obtain from Applied Biosystems the MSDS for any chemical supplied by Obtaining Applied Biosystems. This service is free and available 24 hours a day.

  • Page 22: Chemical Waste Safety

    • Minimize contact with chemicals. Wear appropriate personal protective equipment when handling chemicals (for example, safety glasses, gloves, or protective clothing). For additional safety guidelines, consult the MSDS. xxii Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 23: Electrical Safety

    FIRE HAZARD. For continued protection against the risk of fire, replace fuses only with fuses of the type and rating specified for the instrument. xxiii Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 24: Physical Hazard Safety

    Microbiological and Biomedical Laboratories (stock no. 017-040-00547-4; http://bmbl.od.nih.gov) • Occupational Safety and Health Standards, Bloodborne Pathogens (29 CFR§1910.1030; http://www.access.gpo.gov/nara/cfr/ waisidx_01/29cfr1910a_01.html). Additional information about biohazard guidelines is available at: http://www.cdc.gov xxiv Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 25: Workstation Safety

    Laboratory Use, Part 1: General Requirements” and EN 61010-2-010, “Particular Requirements for Laboratory Equipment for the Heating of Materials,” and with standard EN 61010-2-081:2002+A1:2003 “Particular Requirements for Automatic and Semi-Automatic Laboratory Equipment for Analysis and Other Purposes.” Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 26 This instrument has been tested to and complies with standard AS/NZS 2064, “Limits Australian EMC and Methods Measurement of Electromagnetic Disturbance Characteristics of Industrial, Standards Scientific, and Medical (ISM) Radio-frequency Equipment.” xxvi Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 27: Before Starting The Installation

    Performing the Background See page 5 Calibration and Planning the Installation Optical Calibration Performing the Pure Dye Calibration Verifying the Instrument Performance Powering Off the 7300/7500/ 7500 Fast System Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 28: Setting Up The Computer

    • Uncrating and placing the instrument • Checking the shipped materials • Performing an ROI calibration • Performing a background calibration • Performing an optical calibration (7500/7500 Fast systems only) Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 29 99.7% confidence level. the instrument Access the wizard by selecting Program Files 7300/7500/7500 Fast System Real-Time System Setup Wizard. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 30 Recalibrate the wizard automates the recalibrations, and eliminates the need for manual inspection System of pure dye spectra (Chapter 6) and manual calculation of system verification values (Chapter Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 31: Planning The Installation

    Planning the Installation Planning the Installation You can install, calibrate, and validate the Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System within one 8-hour work day. The installation does not require your participation at all times. However, plan to spend most of your time working with the instrument.
  • Page 32 Analyze the TaqMan RNase P plate data. See Note below Note: You must be present at the instrument for approximately 5 minutes of the total run time. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 33: Unpacking And Setting Up The Instrument

    Calibration and Optical Calibration Setting Up the See page 20 7300/7500/7500 Fast Instrument Performing the Pure Dye Calibration Verifying the Instrument Performance Powering Off the 7300/7500/ 7500 Fast System Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 34: Before You Begin

    Before you begin installing the Applied Biosystems 7300/7500/7500 Fast Real-Time Getting Started PCR System: • Set up the computer as described in the Applied Biosystems Real-Time PCR System Computer Setup Guide • Read the Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Site Preparation Guide (P/N 4378654) and complete the preinstallation checklist contained in the guide.

  • Page 35: Selecting Additional Hardware And Software

    Uninterruptable (UPS), especially in areas prone to power failure. Power failures and other events that Power Supply abruptly terminate the function of the 7300/7500/7500 Fast system can corrupt data and (UPS) possibly damage the system. UPSs have finite battery lives, and, consequently provide power for a IMPORTANT! limited time (from 30 minutes to several hours).
  • Page 36 If your 7300/7500/7500 Fast system includes a laptop or tower computer, then the CD-RW drive of the computer can serve as the backup storage device for your system.
  • Page 37 Chapter 2 Unpacking and Setting Up the Instrument Selecting Additional Hardware and Software If you plan to install a firewall or encryption utility to protect your 7300/7500/7500 Fast Security Software system on a network, confirm that the security software does not interfere with USB (Firewall and communication between the 7300/7500/7500 Fast instrument and the SDS software.

  • Page 38: Uncrating And Placing The 7300/7500/7500 Fast System

    • Do not lift an object and twist your torso at the same time. • Coordinate your intentions with your assistant before lifting and carrying. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 39 Keeping your back straight, lift with your legs and place the instrument onto the bench. Place the instrument on the bench next to the computer Bend at knees as shown below. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 40: Checking The Shipped Materials

    • 7300/7500/7500 Fast instrument ® • Dell Laptop Computer 7300/7500/7500 Fast instrument with 7300/7500/7500 Fast instrument with ® ® Dell tower computer and flat-screen monitor Dell laptop computer Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 41 Plate Tool Plate Tool Applicator 4348209 Miscellaneous items (business card –– holder, reply card for installation Splash-Free quality, warranty card, release notes) Support Base Optical Adhesive Covers and Applicator Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 42 Support Base • Paper, Fluorescent Green (not 4323077 Optical Adhesive Covers and Applicator shown) Miscellaneous items (business –– card holder, Reply card for installation quality, warranty card, release notes) Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 43 – Background Plate – Pure Dye Plates (CY3, CY5, FAM, JOE, NED, ROX, SYBR Green, TAMRA, TEXAS RED, and VIC dyes) – ROI Calibration Plate continued on next page Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 44 Amperase UNG (1.25 mL) ® • TaqMan Gene Expression Assay, Eukaryotic 18S rRNA (0.3 mL) • Human Raji cDNA, (25 ng/µL) Genomic Assays Catalog CD 4362363 (not shown) Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 45 • 7300 System RT PCR RQ 4350814 Study • 7500 System 4350819 • 7500 Fast System 4363619 Miscellaneous items (Setup and — Maintenance Video CD, Mouse Pad, Customer Letter, Registration Card) Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 46: Setting Up The 7300/7500/7500 Fast Instrument

    • Phillips screwdriver (small and thin) Materials Required • Power cord (from packing kit) Prepare the installation site as described in the Applied Biosystems 7300/7500/7500 Fast Preparing the Site Real-Time PCR System Site Preparation Guide. Refer to this guide for weights, dimensions, and electrical requirements.

  • Page 47: Applied Biosystems 7300/7500/7500 Fast Real-Time Pcr System Installation And Maintenance Guide

    Remove the packaging plate from the tray and set it aside. Do not discard the packaging plate. Use it Note: Remove for long-term shutdown of the system (see page 104). Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 48 If you have additional hardware that you want to install (see page 9), do so now. Do not connect the USB cable to the 7300/7500/7500 Fast instrument or computer at this time. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 49: Installing The Software And Powering On The Instrument

    See page 30 Function Test Performing the Pure Dye Calibration See page 28 Troubleshooting See page 32 Verifying the Instrument Performance Powering Off the 7300/7500/ 7500 Fast System Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 50: Overview

    Required SDS Software and Powder-free gloves Safety glasses Documentation CDs If you plan to configure the 7300/7500/7500 Fast system computer with IMPORTANT! additional software (see page 10), complete the installation of the 7300/7500/7500 Fast system as described in this guide before installing other software. Installing third-party software to the 7300/7500/7500 Fast system computer before completing the procedures in this manual can complicate the installation.

  • Page 51: Installing The Sds Software For The 7300/7500/7500 Fast System

    CD drive, then double-click SystemSetupWizard.exe. Click Install the SDS Software. Click In the Software Installation Materials Required page, select the I have obtained the materials listed above check box, then click Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 52 Click In the Choose Destination Location page, click to accept the default location. Click In the New Installation page, click start installing the software. Click Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 53 In the Real-Time System Setup wizard Software Installation page, click In the Real-Time System Setup wizard Welcome page, click Continue with “Powering On the Instrument” on page Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 54: Powering On The Instrument

    • USB connector on the back left of the instrument • Either USB port on the computer Tower Computer USB Connection Laptop Computer USB Connection GR2374 7300/7500 electrical with laptop Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 55 • When the installation is complete, the Found New Hardware message box indicates that the hardware is ready to use. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 56: Performing The Instrument Function Test

    In the New Document wizard, click accept the default parameters. If an ROI error is displayed, click . You perform the ROI calibration after you perform the Function test. Click Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 57 When the software prompts you to save the plate document, click Select In the SDS software, select File Exit. Continue with “Performing the Regions of Interest (ROI) Calibration” on page Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 58: Troubleshooting

    Restart the computer. Windows desktop c. Wait until the Windows desktop appears. d. Power on the 7300/7500/7500 Fast instrument. If the green Power On indicator lights up, installation continues. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 59 4. Perform the Instrument Function Test (see page 30). 5. If the test fails again, contact Applied Biosystems technical support (see page xiii) or your service representative. (continued on next page) Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 60 2. Perform the Instrument Function Test (see page 30). 3. If the test fails again, contact Applied Biosystems technical support (see page xiii) or your service representative. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 61: Performing The Regions Of Interest (Roi) Calibration

    Calibration and See page 44 Optical Calibration Calibration Performing the Pure Dye Calibration Verifying the Troubleshooting See page 48 Instrument Performance Powering Off the 7300/7500/ 7500 Fast System Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 62: Overview

    • After replacing the lamp. After every ROI calibration, you must perform a background calibration, IMPORTANT! optical calibration (7500/7500 Fast systems only), pure dye calibration, and instrument verification. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 63 ROI calibration image Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 64: Preparing The Roi Calibration Plate

    Wear powder-free gloves when you handle the ROI Preparing the IMPORTANT! calibration plate. Plate In the desktop, double-click the icon for the SDS software ( ) to start the software. Double-click Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 65 (Remaining steps apply to both standard and Fast <1500× g 00:00:05 plates.) Briefly centrifuge the ROI calibration plate in a centrifuge with a plate adapter (<1500xg). The plate must be well mixed and IMPORTANT! centrifuged. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 66 Close the tray. Apply pressure to the right side of the tray and at an angle. Continue with “Creating a Plate Document for the ROI Calibration” on page Press here Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 67: Creating A Plate Document For The Roi Calibration

    Do not show me this message Click again, then select Never again on this machine. If an ROI error is displayed, click proceed with ROI calibration. Click Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 68 Creating a Plate Document for the ROI Calibration In the SDS software, select Instrument Calibrate. Select In the warning dialog box, click lower the sample block. The ROI Inspector dialog box opens. Click ROI Inspector Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 69 In the ROI Inspector dialog box, set the lamp control: Click Select Idle from the Lamp Control drop-down list (sets the lamp to lower voltage for ROI calibration). Continue with “Performing the ROI Calibration” on page Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 70: Performing The Roi Calibration

    Exposure Time by half, then click Over-saturated ROI image . Repeat until you obtain an acceptable ROI image. If you cannot obtain an acceptable image, see “Troubleshooting” on page Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 71 The software saves the newly generated ROI calibration for Filter A. “OK” appears next to Click Filter A in the Masks Loaded section of the ROI Inspector window. Displays OK when save is complete Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 72 Press the tray door to open it. If you cannot open the tray door, the Note: sample block may be in its raised position, locking the tray position. To lower the block, click Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 73 (see page 49), an optical calibration (7500 systems only, page 59), and pure dye calibrations (see page 67), and instrument verification (see page 87). Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 74: Troubleshooting

    • Check that the heated cover assembly is pulled all the way forward to ensure that the tray can be pushed in properly. Heated cover assembly GR2482 GR2482 7500/7500 fast heated cover assembly Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 75: Performing The Background Calibration And Optical Calibration

    Performing the Optical Calibration See page 59 Calibration for the 7500/7500 Fast System Verifying the Instrument Performance Troubleshooting See page 64 Powering Off the 7300/7500/ 7500 Fast System Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 76: Overview

    • Performs continuous reads of a background plate containing PCR buffer for 10 minutes at 60 °C. • Averages the spectra recorded during the run and extracts the resulting spectral component to a calibration file. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 77 • Handle the calibration plates with care to prevent contamination. Do not place plates on a lab bench, which may contaminate the plate. Always put calibration plates back into their original bags. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 78: Preparing The Background Calibration Plate

    (Remaining steps apply to both standard and Fast <1500× g 00:00:05 plates.) Briefly centrifuge the background plate in a centrifuge with a plate adapter (<1500xg). The plate must be well mixed and IMPORTANT! centrifuged. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 79 • Not centrifuged for enough time cause an abnormally high background signal. Continue with “Creating a Plate Document for the Background Calibration” on page Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 80: Creating A Plate Document For The Background Calibration

    If the Save in field does not display SDS Documents, navigate to D: drive Applied Biosystems SDS Documents. Should be Click SDS Documents Continue with “Performing the Background Calibration” on page Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 81: Performing The Background Calibration

    Before starting the run, the instrument Note: may pause (up to 10 minutes) to allow the heated cover to reach the correct temperature. Continue with “Analyzing the Background Calibration Data” on page Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 82: Analyzing The Background Calibration Data

    , then troubleshoot the failed run. See unsuccessful. The software stopped the extraction “Troubleshooting” on page because one or more raw spectra exceed the maximum limit for the 7300/7500/7500 Fast system. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 83: Analyzing The Background Calibration Data

    FSU, the background plate or the sample block could contain a fluorescent contaminant. Determine the source of the contamination. See “Troubleshooting” on page Select File Close. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 84 Continue with: • 7300 system – “Performing the Pure Dye Calibration” on page • 7500/7500 Fast system – “Performing the Optical Calibration for the 7500/7500 Fast System” on page Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 85: Performing The Optical Calibration For The 7500/7500 Fast System

    ROI calibration plate Materials Required The optical calibration compensates for the physical effects of the additional filter Purpose of the present in 7500/7500 Fast instruments that is not present in the 7300 instrument. 7500/7500 Fast System Optical Calibration Perform an optical calibration:...
  • Page 86 Liquid is • Not centrifuged with enough force, positioned at bottom of well. • Not centrifuged for enough time Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 87 If the Save in field does not display SDS Documents, navigate to D: drive Applied Biosystems SDS Documents. Should be Click SDS Documents Continue with “Performing the Optical Calibration” on page Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 88 Before starting the calibration, the Note: instrument may pause (up to 10 minutes) to allow the heated cover to reach the correct temperature. When the run is complete, click Click Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 89 Do not discard the ROI calibration plate. If you store the plate in its IMPORTANT! original packaging sleeve, you can use the plate up to three times after you open it. Continue with “Performing the Pure Dye Calibration” on page Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 90: Troubleshooting

    Signals that exceed 72,000 fluorescent standard units (FSU) are considered beyond the limit of normal background fluorescence for a 7300/7500/7500 Fast instrument. Such signals may indicate that either the background or the sample block contains fluorescent contaminants. Common contaminants include: ink residue from permanent pens, powder from disposable gloves, and dust.
  • Page 91 7-9, you can determine the location of the contaminated well (B9). Contaminated 5. Repeat step 4 until you identify the location of each well contaminated well. Contaminated well Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 92 Applied Biosystems technical support or your service representative for further assistance. – Absent, then the sample block is contaminated. Decontaminate the sample block (see “Decontaminating the Sample Block” on page 108). Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 93: Performing The Pure Dye Calibration

    Analyzing the Pure Dye Pure Dye See page 77 Calibration Data Calibration Verifying the Instrument Performance Evaluating Pure Dye See page 80 Calibration Spectra Powering Off the 7300/7500/ 7500 Fast System Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 94: Overview

    You must perform a background run before every pure dye calibration. IMPORTANT! Because the age and use of instrument components can affect pure spectra readings, Applied Biosystems recommends performing a pure dye calibration at least every six months. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 95 When you save a plate document after analysis, the software stores the pure spectra file with the collected fluorescence data for that experiment. The Applied Biosystems Real-Time PCR Systems use the following dye sets for Dye Sets calibration: ™...
  • Page 96 Chapter 6 Performing the Pure Dye Calibration Overview The 7300/7500/7500 Fast instrument supports the detection of custom pure dyes (dyes Custom Dyes other than those provided by Applied Biosystems). Custom dyes must fluoresce within the spectral range measured by the instrument: •...

  • Page 97: Preparing For The Calibration

    Prolonged exposure to light can diminish the fluorescence signal strength of the plate. Continue with “Creating a Plate Document for the Pure Dye Calibration” on page Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 98: Creating A Plate Document For The Pure Dye Calibration

    The Pure Spectra Calibration Manager is displayed. Continue with “Preparing Plates and Performing the Pure Dye Calibration” on page Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 99: Preparing Plates And Performing The Pure Dye Calibration

    Standard plates only: Vortex the plate for 5 seconds. Do not vortex Fast plates. (Remaining steps apply to both standard and Fast plates.) Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 100 In the dialog box that prompts you to load the plate (see step 1d on page 73, click , then wait for the run to complete (~5 minutes). > 00:05:00 Click Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 101 (JOE, NED, ROX, SYBR Green, TAMRA, VIC). If you are using a 7500 or 7500 Fast Note: instrument, also perform calibrations for the CY3, CY5, and TEXAS RED pure dyes. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 102 Preparing Plates and Performing the Pure Dye Calibration After you calibrate the instrument with all pure dyes provided in your spectral calibration kit, click Click Continue with “Analyzing the Pure Dye Calibration Data” on page Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 103: Analyzing The Pure Dye Calibration Data

    Click (or select Analysis Extract Pure Spectra). The SDS software completes the extraction, then Click displays a message (see next page). Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 104 In the pure dye plate document: Select the Results tab. Select the Spectra tab. Select all wells of the plate document by clicking Click the upper-left corner of the plate grid. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 105 The variations in spectral position and peak position are caused by minor differences in the optical and excitation energy between individual wells. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 106: Evaluating Pure Dye Calibration Spectra

    If the peak for the spectra of the dye occurs in the wrong filter, you may have run the wrong dye plate during the calibration. Repeat the procedures for only that dye. Table 1 Spectra for pure dyes supported by the 7300 system Instrument...
  • Page 107 Chapter 6 Performing the Pure Dye Calibration Evaluating Pure Dye Calibration Spectra Table 1 Spectra for pure dyes supported by the 7300 system (continued) Instrument Pure Dye Filter Peak (nm) 7300 System ~610 nm SYBR ~520 nm Green TAMRA ~580 nm...
  • Page 108 Table 2 Spectra for pure dyes supported by the 7500 system Instrument Pure Dye Filter Peak (nm) 7500 System ~520 nm ~550 nm ~550 nm ~610 nm SYBR ~520 nm Green Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 109 Table 2 Spectra for pure dyes supported by the 7500 system (continued) Instrument Pure Dye Filter Peak (nm) 7500 System TAMRA ~580 nm ~550 nm ~580 nm ~670 nm TEXAS RED ~610 nm Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 110 Table 3 Spectra for pure dyes supported by the 7500 Fast system Instrument Pure Dye Filter Peak (nm) 7500 Fast System ~520 nm ~550 nm ~550 nm ~610 nm SYBR ~520 nm Green Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 111 Table 3 Spectra for pure dyes supported by the 7500 Fast system (continued) Instrument Pure Dye Filter Peak (nm) 7500 Fast System TAMRA ~580 nm ~550 nm ~580 nm ~670 nm TEXAS RED ~610 nm Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 112 Chapter 6 Performing the Pure Dye Calibration Evaluating Pure Dye Calibration Spectra Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 113: Verifying The Instrument Performance

    Pure Dye ® Analyzing the TaqMan See page 94 Calibration RNase P Plate Data Verifying the Instrument Performance Troubleshooting See page 99 Powering Off the 7300/7500/ 7500 Fast System Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 114: Overview

    (7500/7500 Fast systems only) pure dye instrument verification). • After moving the instrument to another location. • As needed to verify the function of the 7300/7500/7500 Fast instrument. The TaqMan RNase P Instrument Verification Plate run verifies the performance of an Purpose of Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System.
  • Page 115 Up to six outlier wells from each unknown replicate group in a 96-well IMPORTANT! TaqMan RNase P Instrument Verification Plate can be omitted from the analysis to meet specifications. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 116: Preparing The Taqman Rnase P Verification Plate

    (Remaining steps apply to both standard and Fast plates.) Centrifuge the plate for 2 minutes in a centrifuge with a plate adapter (<1500xg). <1500× g 00:00:05 The plate must be well mixed and IMPORTANT! centrifuged. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 117 “Creating a Plate Document for the RNase P Verification Run” on page Liquid is at • Not centrifuged with enough force, bottom of well. • Not centrifuged for enough time Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 118: Creating A Plate Document For The Rnase P Verification Run

    Documents, navigate to D: drive Applied Should be Biosystems SDS Documents. SDS Documents After selecting the folder, Click Prepare and run the RNase P plate as explained page Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 119: Performing The Verification Run

    (up to 10 minutes) to allow the heated cover to reach the correct temperature. When the run is complete, click ® Continue with “Analyzing the TaqMan RNase P Plate Data” on page Click Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 120: Analyzing The Taqman Rnase P Plate Data

    The SDS software analyzes the run data and Select displays the results in the Results tab. Access the results. In the plate document: Select the Results tab. Select the Amplification Plot tab. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 121 96-well plate. Beginning at well A1, the wells are numbered from left-to-right, and top-to-bottom. Select View Well Inspector. Outliers 7300/7500 system plate Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 122 In the Results tab, select the Standard Curve tab. Select In the Standard Curve tab, verify that the R2 value is ≥0.990. Must be ≥0.99 In the Results tab, select the Report tab. Select Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 123 • If the 10K value is not greater than the 5K, the 7300/7500/7500 Fast system has failed the installation verification. Troubleshoot the RNase P verification run. See “Troubleshooting” on page Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 124 Remove the RNase P plate: Press the tray to open it. Remove the RNase P plate. Press the tray to move it into the instrument. Discard the plate. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 125: Troubleshooting

    TaqMan RNase P Instrument Verification Plate. If the replacement RNase P plate fails, contact Applied Biosystems technical support or your service representative for further assistance. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 126 Chapter 7 Verifying the Instrument Performance Troubleshooting Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 127: Powering Off The 7300/7500/7500 Fast System

    Background Calibration and Optical Calibration Performing a Long-Term See page 104 Shutdown Performing the Pure Dye Calibration Verifying the Instrument Performance Powering Off the 7300/7500/ 7500 Fast System Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 128: Performing A Short-Term Shutdown

    Press the tray to open it. If the tray contains a plate, remove it, then press the tray to move it into the instrument. Press the instrument power button. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 129 Performing a Short-Term Shutdown Power off the computer and monitor: Select Shut Down. In the Shut Down Windows dialog box (not shown), select Shut Down. Power off the monitor. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 130: Performing A Long-Term Shutdown

    Press the instrument power button. Power off the computer and monitor: Select Shut Down. In the Shut Down Windows dialog box (not shown), select Shut Down. Power off the monitor. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 131: Maintaining The Instrument

    • Perform an optical calibration on 7500/7500 Fast systems (see page Tasks • Clean up and defragment the computer hard drive (see page 114) Su M T W Th F S Month (30 Days) Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 132: Recommended Maintenance Schedule

    115) • Replace the halogen bulb (see page 117) • Replace the instrument fuses (see page 122) • Update the Microsoft Windows operating system (see page 124) Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 133: Archiving And Backing Up Sds Files

    SDS software. During a single day of real-time operation, the Applied Data Biosystems 7300/7500/7500 Fast Real-Time PCR System can generate over 0.5 MB of Management data. Data management is a concern only if you perform absolute or relative Strategy quantification experiments on your 7300/7500/7500 Fast system.

  • Page 134: Decontaminating The Sample Block

    Decontaminating the Sample Block Decontaminating the Sample Block PHYSICAL INJURY HAZARD. Do not remove the instrument cover. There are no components inside the 7300/7500/7500 Fast system that you can safely service yourself. If you suspect a problem, contact an Applied Biosystems Service Representative.
  • Page 135 The ROI Inspector dialog box opens. In the ROI Inspector dialog box, click Power off, then unplug the 7300/7500/7500 Fast system. Allow it to cool for 15 min. >00:15:00 Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 136 Pipette the water to a waste beaker. Using a cotton swab, scrub inside of each contaminated well. Using a lint-free cloth, absorb the excess deionized water. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 137 Exposure causes eye, skin, and respiratory tract irritation and may cause central nervous system depression and liver damage. Read the MSDS, and follow the handling instructions. Wear appropriate protective eyewear, clothing, and gloves. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 138 In each contaminated well, pipette the solution up and down several times to rinse the well. Pipette the bleach solution to a waste beaker. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 139 If contamination is present, contact Applied Biosystems technical support (see page xiii). Ensure that the heated cover assembly door is completely closed. If it is not, an error message is displayed. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 140: Cleaning Up And Defragmenting The Hard Drive

    At the top of the dialog box, select the Volume (hard drive) to defragment. Click When the Defragmentation Complete dialog box displays, click Close. Click Repeat steps 3 through 5 for the remaining drives on the computer. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 141: Moving The 7300/7500/7500 Fast System

    Move your 7300/7500/7500 Fast system according to the guidelines on page Connect the components of the system (see “Setting Up the 7300/7500/7500 Fast Instrument” on page 20). Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 142 Perform an optical calibration if you are using a 7500 or 7500 Fast system (see page 59). Perform a pure dye calibration (see page 67). Perform an instrument verification run (see page 87). Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 143: Monitoring Lamp Status

    Displayed at the start of a run if the lamp usage exceeds exceeded 2000 hours. We 2000 hours. recommend replacing the lamp Click Cancel Run, then replace the lamp, or click Continue soon to ensure optimal assay Run. performance. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 144: Replacing The Halogen Lamp

    Wear powder-free gloves when you handle the lamp. Replacing the IMPORTANT! Lamp Power off, then unplug the 7300/7500/7500 Fast system. Allow the instrument to cool for 15 minutes. >00:15:00 Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 145 Place the new lamp into the instrument: Slide the lamp release lever upward. Firmly grasp the lamp, place it into the slotted mount, then carefully slide the lamp downward into place. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 146 Plug in and power on the 7300/7500/7500 Fast system. In the SDS software, select Instrument Calibrate. Select In the ROI Inspector dialog box, select Lamp Control Idle. Select Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 147 Interest (ROI) Calibration • Chapter 5, Performing the Background Calibration and Optical Calibration • Chapter 6, Performing the Pure Dye Calibration • Chapter 7, Verifying the Instrument Performance Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 148: Replacing The Instrument Fuses

    250V, 5 x 20 mm gloves glasses screwdriver Turn off the instrument, then unplug it. Replacing the Fuses Using a flat-head screwdriver, unscrew and remove the fuse holders from the instrument. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 149 Fuse failure can result from fluctuations in Note: the supplied power to the instrument. To prevent further failures, consider installing an electrical protective device (see page Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 150: Updating The Operating System Software And Service Packs

    Updating the Operating System Software and Service Packs Updating the Operating System Software and Service Packs Do not upgrade the operating system of the computer connected to the 7300/7500/7500 Fast system unless instructed to do so by an Applied Biosystems representative. New versions of the Microsoft Windows operating system can conflict with the SDS software and make the instrument inoperable.

  • Page 151: Appendix B Creating A Background Plate

    Remove an Applied Biosystems 96-Well Optical Reaction Plate or 96-Well Fast Reaction Plate from its box and place it on a clean, dry surface. Aliquot 50 µL (7300/7500 system) or 20 µL (7500 Fast system) of deionized water to each well of the reaction plate.
  • Page 152 Appendix B Creating a Background Plate Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 153: Appendix C Creating A Custom Pure Dye Plate

    Creating a Custom Pure Dye Plate Overview The Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System can be used to run assays designed with custom dyes (dyes not manufactured by Applied Biosystems). Custom dyes must fluoresce within the spectral range measured by the instrument: •...

  • Page 154: Determining Optimum Dye Concentration

    Load the prepared plate: Press the tray door to open it. Load the pure dye plate into the plate holder. Press the tray door to move the drawer into the instrument. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 155 Record the coordinate of the well that displays the brightest possible signal without saturation. This well contains the best concentration of the custom pure dye for Filter A. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 156: Creating A Custom Pure Dye Plate

    Instrument Calibrate, then exit the ROI Inspector. Click Creating a Custom Pure Dye Plate Prepare 5 mL (7300/7500 system) or 2 mL (7500 Fast system) of the custom pure dye at the concentration determined in step 10 on page 130.

  • Page 157: Adding The Custom Dye To The Software

    In the Add Dye dialog box, enter a name for the custom dye, then click Enter a name for Click the dye Run the custom pure dye plate (see Chapter 6, “Performing the Pure Dye Calibration.”) Click Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 158 Appendix C Adding the Custom Dye to the Software Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 159: Setting Up The Computer

    ..... . . 139 ■ Connecting to the Network and Downloading Adobe Acrobat ... . 141 Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 160: Unpacking The Computer And Setting Voltage

    Do not connect components or the USB cable to the instrument or computer at this time. Doing so may result in installation of incorrect drivers. Tower Computer Laptop Computer Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 161 AC adaptor and the laptop computer (you can discard remaining power cords). Outside the US, use the power cord from the international IMPORTANT! voltage kit, not the power cord provided in the computer carton. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 162: Connecting Components And Powering Up

    The power switch on a laptop is at the top-center of the keyboard. Wait for the computer to boot. Log on using an account with windows administrator privileges (see your system administrator for more information). Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 163: Setting The Display Settings And Power Options

    Select Control Panel. In the top left of the Control Panel, click Switch to Classic View. Click In the Control Panel window, double-click Display. Double-click Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 164 768 pixels. Select Color quality Highest (32 bit). Click If you are using a laptop computer and the computer displays a Flat Panel Warning dialog box, click Click Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 165 IMPORTANT! Hibernate power setting is disabled. If the Hibernate setting is enabled, data collection will stop when the computer goes into Hibernate mode. Close the Control Panel window. Notes Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...

  • Page 166: Connecting To The Network And Downloading Adobe Acrobat

    Connect your computer to the network. For help in connecting your computer to the network, see your system administrator. Do not use the 7300/7500/7500 Fast System on a wireless network. IMPORTANT! Use of a wireless network can interfere with data collection and may result in data loss.

  • Page 167: Index

    SDS files DANGER, description backup storage devices data management biohazardous waste, handling xxiii decontaminating the sample block biological hazard guidelines xxiv Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 168 15, software and documentation 15, Spectral Calibration halogen bulb TaqMan Reagent Starter replacing ® TaqMan Reagent Starter warning, hot 118, ® TaqMan RNase P Chemistry hardware, selecting additional Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 169 69, 80, 82, performing background calibration instrument function test optical calibration (7500/7500 Fast systems R2 value only) radioactive waste, handling xxiii pure dye run RNase P run Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 170 RNase P plate standard plate ROI plate description location of notched corner standards safety safety before operating the instrument status lights, front panel biological hazards xxiv Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 171 Error indicator is lit RNase P run failed ROI image is faint Shutter failure Thermal Cycler failure USB failure uncrating the instrument uninterruptable power supply, requirements unloading a plate Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 172 Index Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide...
  • Page 174 Web site at www.appliedbiosystems.com. Applera is committed to providing the world’s leading technology and information for life scientists. Applera Corporation consists of the Applied Biosystems and Celera Genomics businesses. Headquarters 850 Lincoln Centre Drive Foster City, CA 94404 USA Phone: +1 650.638.5800...

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