Applied Biosystems 7900HT User Manual page 321

Format
(for a single well):
Example
Example Code A-12 Allelic discrimination setup table files
*** SDS Setup File Version
*** Output Plate Size
*** Output Plate ID
*** Number of Detectors
Detector Reporter
CYP 2C9*2.1 FAM
CYP 2C9*2.2 VIC
*** Number of Markers
Marker AlleleX
CYP 2C9*2 CYP 2C9*2.2
Well Sample Name
1 Sample 1
2 Sample 2
3 ...
384 Sample 48
Well Sample Name
1 Sample 1
2 Sample 2
3 ...
384 Sample 48
Example Code A-13 Absolute quantification setup table files
*** SDS Setup File Version
*** Output Plate Size
*** Output Plate ID
*** Number of Detectors
Detector Reporter
RNase P FAM
Well Sample Name
1 Sample 1
2 Sample 2
3 ...
96 Sample 30
Example Code A-14 Relative quantification setup table files
*** SDS Setup File Version
*** Output Plate Size
*** Output Plate ID
*** Number of Detectors
Detector Reporter
GAPDH VIC
TNF-α FAM
Well Sample Name
1 Calibrator
2 Sample 1
3 ...
96 Sample 12
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
[ Well number <tab> SDS Sample Name <tab> Marker name <tab> Marker task ]
{ <tab> Marker name <tab> Marker task <tab> ... } [ <cr> ]
3
384
384N75822034
2
Quencher
1
AlleleY
CYP 2C9*2.2
Detector Task
Marker Task
CYP 2C9*2 UNKN
CYP 2C9*2 UNKN
CYP 2C9*2 UNKN
3
96
96JG729FF903
1
Quencher
TAMRA
Detector Task
GAPDH UNKN
GAPDH STND
GAPDH UNKN
3
96
96FRE505SDA2
2
Quencher
Detector Task
GAPDH ENDO
GAPDH ENDO
GAPDH ENDO
Description
PDAR CYP 2C9*2 Al1
PDAR CYP 2C9*2 Al2
Description
PDAR CYP 2C9*2
Quantity
Marker Task
Description
RNase P Probe
Quantity Detector
0
20000
0
Description
GAPDH Probe
TNF-α Probe
Quantity Detector
0 TNF-α
0 TNF-α
0 TNF-α
September 1, 2004 11:39 am, App_SWReference.fm
Setup Table Files
Comments
Example Probe
Example Probe
Comments
Example marker
Comments
Example Probe
Task Quantity
Comments
Example Probe
Example Probe
Task Quantity
UNKN 0
UNKN 0
UNKN 0
DRAFT
A-13