Getting Started - Applied Biosystems 7900HT User Manual

Getting Started

Using the SDS
Software
Online Help
Examples in This
Chapter
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
For specific instructions on any procedure described within this section, refer to the
Sequence Detection Systems Software Online Help. To get help at any time during the
procedure, click located within the dialog box or window in which you are
working.
The screenshots that appear within this chapter were created using a series of
®
TaqMan Cytokine Gene Expression Plates (PN 4304671), a research tool for
real-time, in vitro quantitative evaluation of human cytokine gene expression. The
plate detects the expression of 12 cytokine target sequences and an endogenous
control in complementary DNA (cDNA) samples. The TaqMan Cytokine Gene
Expression Plate I consists of a MicroAmp
into 24 replicate groups, each containing TaqMan probes and primers for the assay of
one human cytokine mRNA and an endogenous control.
assay configurations for the plate.
The study used to produce the screenshots for this section consisted of four plates of
a time-course experiment run to evaluate the expression of 24 target cytokine mRNA
over a 36-hour period.
IL-2
IL-3
IL-6
IL-10
IL-13
IL-18
M-CSF
TGFβ
Figure 6-8 Sample Configuration of the Example Plates
For more information on the TaqMan Cytokine Gene Expression Plate I, see
Note:
the TaqMan Cytokine Gene Expression Plate I Protocol (PN 4306744).
®
Optical 96-Well Reaction Plate arranged
IL-1α IL-1β
IL-4 IL-5
IL-7 IL-8
IL-12p35 IL-12p40
IL-15 IL-17
G-CSF GM-CSF
IFNγ LTβ
TNFα TNFΒ
DRAFT
September 1, 2004 11:39 am, CH_Real-Time.fm
Getting Started
Figure 6-8 illustrates the
36 hr
Plate 1
24 hr
Plate 2
12 hr
Plate 3
0 hr
Plate 4
6-21