Preparing The Background Plate Or Taqman Low Density Array; Creating A Plate Document For The Background Run - Applied Biosystems 7900HT User Manual

Preparing the Background Plate or TaqMan Low Density Array

Preparing a
Background Card
Preparing and
Running a
Background Plate

Creating a Plate Document for the Background Run

Preparing a
Background Plate
Document
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
1. Remove the background Low Density Array from its packaging and set it aside
to warm to room temperature.
2. Remove the tube of background solution from the freezer to thaw. When the
solution has thawed, vortex the tube.
3. Load the background solution into the background Low Density Array, loading
100 µL of solution per fill reservoir.
4. Centrifuge the Low Density Array according to the procedures in
the TaqMan Low Density Arrays" on page
5. Seal the Low Density Array according to the procedures in
TaqMan Low Density Arrays" on page
6. Continue with "Creating a Plate Document for the Background Run"
1. Choose from the following:
If...
using a background
plate from a Spectral
Calibration Kit
creating a
background plate
2. Briefly centrifuge the background plate.
3. Continue with "Creating a Plate Document for the Background Run"
1. Start the SDS software.
2. Click (or select File > New).
3. Configure the New Document dialog box:
• Assay – Select Background.
• Container – Select the appropriate plate format.
• Template – Select Blank Template.
4. If the background plate or Low Density Array is labeled with a bar code, click
the Barcode field, then scan the bar code number using the hand-held bar code
reader.
4-16.
4-19.
remove the plate from the freezer and allow it to thaw to
room temperature.
1. Pipette deionized water to each well of the plate.
– If using a standard 384-well plate, add 20 µL per well.
– If using a standard 96-well plate, add 50 µL per well.
2. Seal the plate using an optical adhesive cover or optical
flat caps.
DRAFT
September 1, 2004 11:39 am, CH_Maintenance.fm
Performing a Background Run
"Centrifuging
"Sealing the
below.
Then...
below.
7-17