Section 4.3 Automated Operation - Applied Biosystems 7900HT User Manual

Section 4.3 Automated Operation

In This Section
Pre-Run
Checklist
Overview
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
Operating the Software with an SDS Enterprise Database . . . . . . . . . . . . . . . . . . 4-32
Operating the Software without an SDS Enterprise Database . . . . . . . . . . . . . . . 4-34
Operating the Software without an SDS Enterprise Database . . . . . . . . . . . . . . . 4-39
After the Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-44
The following tasks must be complete to run plates on the 7900HT instrument. See
the associated page number for details on each procedure.
Done Check
A background run has been performed in the last week
A pure dye run has been performed in the last 6 months
The instrument tray does not contain a plate
IMPORTANT!
the instrument tray contains a plate, you must eject and remove it.
The output stack does not contain plates.
The Applied Biosystems 7900HT Fast Real-Time PCR System features the capacity
for high-throughput unattended operation through the use of the Automation
Controller Software. The Automation Controller Software coordinates the function
of the 7900HT instrument, the fixed-position bar code reader, and the Zymark
Twister Microplate Handler to run batches of prepared plates with minimal user
intervention. The Automation Controller Software can run plates using plate
documents stored locally on the computer attached to the instrument, or across a
network on an SDS Enterprise Database.
Options for Automated Operation
As with the SDS Software, the Automation Controller Software requires the creation
of a plate document for each plate it runs. The Automation Controller Software can
run batches of plates using plate documents saved to the hard drive of the computer
attached to the instrument, or stored on an SDS Enterprise Database.
Follow the appropriate procedure for your system:
Operating the Software with an SDS Enterprise Database . . . . . . . . . . . . . . . . . . 4-32
Operating the Software without an SDS Enterprise Database . . . . . . . . . . . . . . . 4-34
The instrument tray must be empty to begin a run. If
September 1, 2004 11:39 am, CH_Basic.fm

Section 4.3 Automated Operation

DRAFT
See Page
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7-20
4-44
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4-31