Applied Biosystems 7900HT User Manual page 367

Glossary
.sdd
.sdm
.sds
.sdt
∆C
T
∆∆C
T
∆R
n
5' nuclease assay
ABI P SDS
RISM
Detector file (*.sdd)
SDS 7900HT
Multiple Plate
Document (*.sdm)
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
The file extension of an ABI P
7900HT instrument.
The file extension of a
software for the 7900HT instrument.
The file extension of a
7900HT instrument.
The file extension of a
the 7900HT instrument.
The difference between the threshold cycle of a sample assay and the threshold cycle
of the corresponding endogenous reference: ∆C
control).
For a given cDNA target, the difference between the average ∆C
sample and the average ∆C
∆∆C
(test sample) = Avg∆C
T
is used to calculate expression fold value by the equation: Expression fold value =
−∆∆C
2
T
The normalized reporter signal minus the baseline signal. The baseline is established
in the first few cycles of PCR. Like R
PCR when the amplicon copy number increases until the reaction approaches a
plateau.
The 5' (five prime) nuclease assay in combination with the TaqMan
the basis for real-time polymerase chain reaction (PCR) technology on the 7900HT
instrument. See "Fundamentals of the 5´ Nuclease Assay" on page D-2
description of the reaction.
The SDS detector file is a tab-delimited flat file that is used to transfer detector
information to and from the SDS software using the Export and Import functions of
the Detector Manager software. The file contains: (a) a header
"SDS2.2.1[GDF[Global Detector File", (b) tab-delimited data for each detector (allele
Name, the name of the reporter dye, the name of the quencher dye, a description, a
comment, the owner, the creation date and time, the modification date and time, and
the color used to code the detector in graphical displays of the SDS software).
Multiple plate documents are used for the analysis of relative quantification data.
SDS Detector file for SDS software for the
RISM
SDS 7900HT Multiple Plate Document (*.sdm)
SDS 7900HT Document (*.sds)
SDS 7900HT Template Document (*.sdt)
for the corresponding calibrator sample:
T
(test sample) - Avg∆C
T
, ∆R
is not a fixed value but increases during
n n
September 1, 2004 11:39 am, Glossary.fm
for SDS software for the
for SDS software for
= C (target) - C - C
T T T
value of a target
T
(calibrator sample). This value
T
® probes serves as
DRAFT
for SDS
(endogenous
T
for a detailed
Glossary-1