Applied Biosystems 7900HT User Manual page 307

Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
Table 8-6 Troubleshooting the TaqMan
Observation
After pipetting, too little
PCR reaction mixture has
gone into the fill reservoir.
After pipetting, some of the
PCR reaction mixture leaks
out of the vent port in the
fill reservoir.
The PCR reaction mixture
leaks during PCR cycling.
After a mean assay result
and standard deviation of
the replicates are
calculated, a specific
replicate appears to be an
outlier.
® Low Density Array
Possible Cause
The PCR reaction mixture
was not correctly pipetted
into the fill reservoir.
The aluminum foil backing
may have been damaged
during the sealing step.
N/A
DRAFT
September 1, 2004 11:39 am, CH_Trouble.fm
TaqMan Low Density Array
Recommended Action
Care must be taken to
correctly pipette the PCR
reaction mixture (100 µL)
into the fill reservoir.
1. Carefully follow the
procedures provided in
"Loading the TaqMan
Low Density Arrays" on
page 4-14.
2. After centrifuging, be
sure to note the fluid
levels in the fill reservoir
as described in step 6 on
page 4-18.
Discard the Low Density
Array.
Note:
See "About the
Sealer" on page 4-14 for
proper sealing instructions.
Review the multicomponent
analysis display for that
replicate. Delete the outlier
and reanalyze.
8-19