Applied Biosystems ViiA 7 Real Quick Reference Manual

Applied Biosystems ViiA 7 Real Quick Reference Manual

Time pcr system

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ViiA™ 7 Real-Time PCR System
Note:
For safety and biohazard guidelines, refer to the
"Safety" appendix in the Applied Biosystems ViiA
Time PCR System User Guide (PN 4442661). For every
chemical, read the SDS and follow the handling
instructions. Wear appropriate protective eyewear, clothing,
and gloves.

In this guide

Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Calibrate the ViiA
7 System . . . . . . . . . . . . . . . . . . . . . . . . . .
Perform an experiment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Maintain the ViiA
7 System. . . . . . . . . . . . . . . . . . . . . . . . . .
Power off the ViiA
7 System. . . . . . . . . . . . . . . . . . . . . . . . .
For details on operating and maintaining your ViiA
to the Applied Biosystems ViiA
(PN 4442661).
7 System, refer
7 Real-Time PCR System User Guide
Description
7 Real-
The Applied Biosystems ViiA
fluorescent-based polymerase chain reaction (PCR) reagents to
provide:
• Quantitative research detection of target nucleic acid
sequences (targets) using real-time analysis.
• Qualitative research detection of targets using post-PCR
(endpoint) analysis.
1
• Qualitative analysis of the PCR product achieved by melt curve
analysis that occurs post-PCR.
2
5
15
22
QUICK REFERENCE GUIDE
7 Real-Time PCR System uses

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Summary of Contents for Applied Biosystems ViiA 7 Real

  • Page 1 QUICK REFERENCE GUIDE Note: For safety and biohazard guidelines, refer to the Description ™ “Safety” appendix in the Applied Biosystems ViiA 7 Real- ™ The Applied Biosystems ViiA 7 Real-Time PCR System uses Time PCR System User Guide (PN 4442661). For every...
  • Page 2 • 96-Well Region of Interest (ROI) and Background Plates (PN 4432364) ™ ™ ® ™ • 96-Well Normalization Plates with FAM /ROX and VIC /ROX Dyes (PN 4432370) ® • TaqMan RNase P 96-Well Instrument Verification Plate (PN 4432382) ™ Applied Biosystems ViiA 7 Real-Time PCR System Quick Reference Guide...
  • Page 3 Prolonged exposure to light can diminish the fluorescence of the dye. Fill the array card with calibration mix. For a normalization calibration, fill two cards with separate normalization solutions. ™ Applied Biosystems ViiA 7 Real-Time PCR System Quick Reference Guide...
  • Page 4 After the run, view the results in the Analysis tab to verify that the calibration run passed. If the ™ complete the calibration run failed, troubleshoot the failed calibration as described in the Applied Biosystems ViiA 7 Real- Time PCR System User Guide.
  • Page 5 – Array-card staker/sealer fixture – Array card Workflow Design the experiment (page Prepare the reactions (page Start and monitor the experiment (page View analysis reports (page Export results (page ™ Applied Biosystems ViiA 7 Real-Time PCR System Quick Reference Guide...
  • Page 6 Up Standards, select a target, define the standard curve, then select and arrange wells for the standards. • (Optional) (Standard Curve, Relative Standard Curve, and Comparative C ) Assign Biological Replicate Groups to wells. ™ Applied Biosystems ViiA 7 Real-Time PCR System Quick Reference Guide...
  • Page 7 Save the file After you design an experiment, you can also save the experiment as a template, then create experiments from the template using QuickStart. ™ Applied Biosystems ViiA 7 Real-Time PCR System Quick Reference Guide...
  • Page 8 • (Genotyping) Prepare the reactions for each SNP separately • Place the reaction plate or array card at 4 °C in the dark until you are ready to load it into the instrument. ™ Applied Biosystems ViiA 7 Real-Time PCR System Quick Reference Guide...
  • Page 9 ® ™ Twister II Robot or to the sample block of the ViiA 7 System. Note: Go to “Start and monitor the experiment” on page ™ Applied Biosystems ViiA 7 Real-Time PCR System Quick Reference Guide...
  • Page 10 Remove the array card Remove the Applied Biosystems Array Card from the box and place it on a clean, dry surface. Load chemicals and For each chemical and solution transferred: solutions into the array Place the array card on a lab bench, with the foil side down.
  • Page 11 Press down on all four corners of the array card to ensure that it is fully seated within the fixture. Alignment pins Use the two alignment pins in the fixture to position the array card correctly. ™ Applied Biosystems ViiA 7 Real-Time PCR System Quick Reference Guide...
  • Page 12 • Load plates and array cards with the barcode facing the front of the instrument. IMPORTANT! Plates should loaded and unloaded by trained operators that have been warned of the moving parts hazard. Touch to load the plate. ™ Applied Biosystems ViiA 7 Real-Time PCR System Quick Reference Guide...
  • Page 13 To confirm that the r2 value is acceptable Derivative Melt Curve Melt Curve and any other type, when the experiment To review melt curve data includes a melt curve ™ Applied Biosystems ViiA 7 Real-Time PCR System Quick Reference Guide...
  • Page 14 (Optional) Save export settings. Select the Save Export Set As check box and name the settings file. You can select the settings file later when you perform a similar experiment. Click Start Export. ™ Applied Biosystems ViiA 7 Real-Time PCR System Quick Reference Guide...
  • Page 15 7 System 384-Well Sample Block (PN 4453553) Array card sample block • Applied Biosystems ViiA™ 7 System 384-Well/Array Card Heated Cover (PN 4453555) • Applied Biosystems ViiA™ 7 System Array Card Adapter (PN 4454166) • Applied Biosystems ViiA™ 7 System Array Card Sample Block (PN 4453554) 96-well sample block ™...
  • Page 16 CAUTION! Before using a cleaning or decontamination method other than those recommended by the Applied Biosystems, verify with Applied Biosystems that the proposed method will not damage the equipment.
  • Page 17 ™ Plug in, then power on the ViiA 7 System. ™ Applied Biosystems ViiA 7 Real-Time PCR System Quick Reference Guide...
  • Page 18 (step 2) to rinse the wells of the sample block. Perform a background calibration, to verify that you have eliminated the contamination. If the contamination remains, contact Applied Biosystems Technical Support. ™ Applied Biosystems ViiA 7 Real-Time PCR System Quick Reference Guide...
  • Page 19 If the indicator is on the red line, push the sample ™ block into the ViiA 7 System until it is seated correctly. ™ Applied Biosystems ViiA 7 Real-Time PCR System Quick Reference Guide...
  • Page 20 In the Main Menu, touch ™ Applied Biosystems ViiA 7 Real-Time PCR System Quick Reference Guide...
  • Page 21 The installation is successful if the instrument powers on and if the Block Type field displays the correct type of sample block. Perform calibrations Perform calibrations in the order shown in “A note on calibration order” on page ™ Applied Biosystems ViiA 7 Real-Time PCR System Quick Reference Guide...
  • Page 22 Use the touchscreen to perform a self test of the ViiA 7 System. After the self test is complete, the instrument generates a detailed summary of the results. You can save the summary to a USB drive and email them to Applied Biosystems Technical Support. ™...

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