Decontaminating The Sample Block - Applied Biosystems 7900HT User Manual

Chapter 7 Maintaining the Instrument

Decontaminating the Sample Block

When to Perform
Materials
Required
Cleaning a Low
Density Array
Block
7-14 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, CH_Maintenance.fm
The following procedure describes how to decontaminate the wells of a sample block
module. The procedure eliminates residual PCR-related products, including
fluorescent labeled TaqMan
If you plan to use a cleaning or decontamination method other than
IMPORTANT!
the one in this manual, check with Applied Biosystems first to ensure that the
method will not damage the sample block module or the 7900HT instrument.
• 10% Sodium hypochlorite (bleach) solution
• Isopropanol, 100 percent pure
• 5/32 in hex key
• Cotton swabs
CHEMICAL HAZARD. Sodium hypochlorite (bleach) is a
liquid disinfectant that can be corrosive to the skin and can cause skin
depigmentation. Please read the MSDS, and follow the handling instructions. Wear
appropriate protective eyewear, clothing, and gloves.
CHEMICAL HAZARD. Isopropanol is a flammable liquid and
vapor. It may cause eye, skin, and upper respiratory tract irritation. Prolonged or
repeated contact may dry skin and cause irritation. It may cause central nervous
system effects such as drowsiness, dizziness, and headache, etc. Please read the
MSDS, and follow the handling instructions. Wear appropriate protective eyewear,
clothing, and gloves.
This procedure applies to the sample block on the 7900HT System TaqMan Low
Density Array Upgrade.
1. Wipe the surface of the sample block (grey aluminum) with 10% bleach.
2. Wipe the sample block three times with distilled water.
3. Wipe the sample block with isopropanol, then allow to air dry.
4. Replace the block as explained in
5. Run a background Low Density Array to confirm that the contamination has
been removed.
DRAFT
® probes. Clean the sample block as often as needed.
Circuitry and connections to
the instrument (Do Not Touch)
Warning
Well A-1
"Replacing the Sample Block" on page 7-10.