Before You Begin - Applied Biosystems 7900HT User Manual

Chapter 4 Operating the Instrument

Before You Begin

Pre-Run
Checklist
Workflow
Overview
4-4 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, CH_Basic.fm
The following tasks must be complete to run plates on the 7900HT instrument. See
the associated page number for details on each procedure.
Done Check
A background run has been performed in the last month
A pure dye run has been performed in the last 6 months
The instrument tray does not contain a plate
IMPORTANT!
the instrument tray contains a plate, you must eject and remove it.
If using an Automation Accessory, also check the following...
The output stack does not contain plates.
1. Prepare your experiments for use on the 7900HT instrument:
– Prepare optical plate(s) (see
– Prepare TaqMan
2. Prepare and run the 7900HT instrument:
– Stand-alone Operation (see
– Automated Operation using the SDS Enterprise Database (see
– Automated Operation without the database (see
3. Analyze the run data.
– Allelic Discrimination Analysis (see
– Absolute Quantification Analysis (see
– Relative Quantification Analysis (see
– Dissociation Curve Analysis (see
DRAFT
The instrument tray must be empty to begin a run. If
page 4-8).
® Low Density Array(s) (see
page 4-23).
page 5-5).
page
page
page 6-37).
page 4-10).
page
page 4-34).
6-5).
6-15).
See Page
7-16
7-20
4-44
–
4-32).