Applied Biosystems 7900HT User Manual page 212

Chapter 6 Analyzing Real-Time Data
Viewing Relative
Quantities in the
Analysis Table
6-34 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, CH_Real-Time.fm
The software displays the results of the relative quantification calculation in the
Results table of the plate document.
table containing the data from a TaqMan Human Cytokine Plate I.
s
Figure 6-13 Analysis Table of the Absolute Quantification Plate Document
The columns of the table are:
• Position – Displays the plate the sample is from and the position on the plate
(well number)
• Sample – Displays the name assigned to the well/sample when the associated
plate was run
• Detector – Displays the name of the detector assigned to the sample/well
position
• Task – Displays the task applied to the detector assigned to the sample/well
position
• C – Displays the average calculated threshold cycle for the replicate group
T
associated with the test sample.
The software displays Undetermined in the C
Note:
associated sample fails to produce a noticeable rise in fluorescent signal during
the entire PCR (the fluorescent signal produced by the well never crosses the
threshold level defined for the associated detector).
• ∆C
– Displays the normalized threshold cycle for the sample
T
• Avg∆C – Displays the averaged normalized threshold cycle for the sample
T
replicate group
• ∆C SD – Displays the number of standard deviations from the average ∆C
T
the replicate group that the sample's individual ∆C
• ∆∆C – Displays the calculated ∆∆C
T
with the test sample.
• RQ – Displays the calculated relative level of gene expression for the replicate
group associated with the test sample.
• RQ min – Displays the minimum calculated relative level of gene expression in
the test samples
• RQ max – Displays the maximum calculated relative level of gene expression
in the test samples
• Status – When autocalling is enabled and a sample is reject by the algorithm,
the status field indicates how the sample failed the analysis.
• Rejected – When autocalling is enabled, indicates if the sample data was rejected.
DRAFT
Figure 6-13 shows an example of the results
column when the
T
value lies
T
value for the replicate group associated
T
of
T