Applied Biosystems 7900HT User Manual page 264

Chapter 7 Maintaining the Instrument
Preparing the
Verification Plate
or TGF-β Card
7-32 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, CH_Maintenance.fm
Preparing a TaqMan RNase P Instrument Verification Plate
1. Remove the TaqMan RNase P Instrument Verification Plate from the freezer
and allow it to thaw to room temperature.
2. Briefly centrifuge the TaqMan RNase P Instrument Verification Plate.
3. Continue with "Preparing the Plate Document" on page
Preparing a TGF-β Card
4. Remove the TGF-β card from the refrigerator and allow it to warm to room
temperature.
5. In a sterile 2-mL tube, prepare the following PCR reaction mixture:
Reagent
TaqMan 2✕ Universal PCR Master Mix
Human cDNA
RNase/DNase-free water
Total Volume
6. Tap the tube gently to mix.
7. Pipette 100 µL of the reaction mix into each of the eight fill reservoirs.
8. Continue with "Preparing the Plate Document" on page
DRAFT
7-33.
Volume (µL)
500
50
450
1000
7-33.