Applied Biosystems 7900HT User Manual page 42

Chapter 1 Product Overview
GR2009
7900HT
Front view with Robot
7900HT FAST Real-Time PCR System
GR2009
Applied Biosystems 7900HT
Fast Real-Time PCR System
Instrument Firmware
3
Thermal Cycling and
Sequence Detection
4
Data Collection
1-18 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:38 am, CH_Overview.fm
Automated Operation
In this mode, the Applied Biosystems 7900HT Fast Real-Time PCR System uses an
Automation Accessory (Zymark Twister Microplate Handler and fixed-position bar
code reader), to provide high-throughput operation suitable for small- to
medium-scale studies. With this configuration
batches of plates unattended using the Automation Controller Software. As in
Stand-Alone operation, the computer stores all data for operation of the instrument
and provides the software for analyzing the run data.
Computer
Automation Controller
Software
2
5
Serial
Cable
Figure 1-12 Automated Operation of the of the 7900HT Instrument
Database Operation
For this mode, the Applied Biosystems 7900HT Fast Real-Time PCR System uses
the SDS Enterprise Database and the Automation Accessory, to provide maximum
high-throughput potential. In this configuration
7900HT instrument has the storage capacity and analysis capability to support
medium- to large-scale gene expression and genotyping studies. Moreover, the SDS
Enterprise Database functions as a repository for all SDS-related data produced by
the networked client computers used for data collection and analysis.
DRAFT
Plate document files
added to and run from
the plate queue
Raw Data Saved to
the Plate Documents
(Figure 1-12), a technician can run
SDS Software
1
Plate Documents
Created
Hard drive(s)
*.sds
*.sdt
files
file
6
Plate Document
Opened and Analyzed
(Figure 1-13 on page 1-19), the