Applied Biosystems 7900HT User Manual page 90

Chapter 3 Preparing a Run
Relative
Quantification
Workflow
Dissociation
(Melting) Curve
Workflow
3-6 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, CH_Run.fm
1. Create a relative quantification plate document (see
2. * Apply detectors to the plate document:
a. Create detectors for the relative quantification probes (see
b. Copy the detectors to the plate document (see
3. * Configure the plate document with detector tasks (NTC and Unknown)
(see page 3-16).
4. * Program the method for the relative quantification run (see
5. Choose from the following:
– If running a single plate, then continue to
– If running the first plate in a series of plates with identical assay
configurations, then save the plate document as a template (see
6. Create a plate document from the template created in
7. Configure the document with sample names and plate information (see
page 3-28).
8. Prepare and run the relative quantification plate or plates (see
9. Analyze the run data (see
*Steps 2 and 2 can be eliminated by importing the plate document setup information
from a tab-delimited text file. See
on page A-2 for more information.
The SDS software can perform a dissociation curve analysis as part of an absolute
quantification run. Therefore, to perform a dissociation curve, construct a plate
document for absolute quantification as explained on
method with a temperature ramp as explained on
DRAFT
page
step 2.
page 6-15).
"Importing Plate Document Setup Table Files"
page
page 3-9).
page 3-11).
3-13).
page 3-19).
page 3-24).
step 2 (see page 3-26).
page 4-1).
page 3-5 and configure the
3-23.