Applied Biosystems 7900HT User Manual page 372

quality value
quantity
quantity mean
quantity standard
deviation
quencher dye
ramp
(temperature ramp)
raw data
reaction device
real-time
run/analysis
relative
quantification
reporter dye
Glossary-6 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, Glossary.fm
The quality value for a given point is an estimate of the probability that the assigned
genotype for that point is correct, with respect to the rest of the points in the data set.
The term quality value is only used for allelic discrimination autocalling.
(1) the quantity values for the standards contained on a plate (2) the initial sample
quantity computed for the unknowns contained on a plate.
Statistical mean of the normally distributed quantity values for a replicate group
(wells with the same sample name, detector, and task). Calculated as the sum of all
quantity observations (readings) in the replicate group divided by the number of
replicates.
Statistical standard deviation of the normally distributed quantity values for a replicate
group (wells with the same sample name, detector, and task). The standard deviation
is calculated using the nonbiased or "n-1" method (square root of sum of the squared
normed residuals divided by n-1 degrees of freedom, with n being the number of
replicates.
The fluorescent dye (or non-fluorescent) banned to the 3' end of the TaqMan
which reduces fluorescence of the reporter dye at the 5' end by FRET until the reporter
dye is cleared during PCR.
The interval between two set-points during which the instrument heats or cools the
samples.
Unmodified spectral data detected between the wavelengths of 500 nm and 660 nm
collected by the 7900HT instrument during a real-time or end-point run.
The physical device used to hold the PCR reactions during evaluation on the
Applied Biosystems 7900HT Fast Real-Time PCR System. Examples of reaction
formats include ABI P
TaqMan ® Low Density Array.
Refers to real-time PCR processing of individual plates by the 7900HT instrument for
absolute and relative quantification. The 7900HT instrument collects fluorescence
data during each cycle of a pre-programmed PCR run. The pre-programming occurs
by setting the thermal cycling parameters of a default method. The step parameters
(time and temperature) are adjusted, hold, cycle set, or step are selected, and
optionally, auto increment values, ramp rate values and data collection options can be
configured.
A process by which the quantity of a nucleic acid target, relative to a control target, in
one sample is calculated relative to the quantity in another sample. For example, the
quantity of c-myc in a brain tissue sample relative to the quantity of c-myc in liver
tissue sample.
Fluorescent dye used to bind to the 5' end of the TaqMan
of dyes, such as FAM
DRAFT
™ 384- and 96-Well Optical Reaction Plates or the
RISM
™ and VIC ® that covalently bind to the 5' end of the probe.
® Probe,
® Probe. There are a number