Applied Biosystems 7900HT User Manual page 98

Chapter 3 Preparing a Run
Creating Markers
for Allelic
Discrimination
3-14 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, CH_Run.fm
Allelic discrimination plate documents feature the use of 'markers' to aid in
organizing and applying detectors based on the loci they target. A marker is a pairing
of two detectors representing chemical assays designed to discriminate between
different alleles of a common locus. The SDS software uses marker information
during data analysis to organize and compare the processed run data.
Allelic discrimination plate documents must contain at least one
IMPORTANT!
marker.
1. If the Detector Manager dialog box is open, click
2. Select Tools > Marker Manager.
3. In the Marker Manager dialog box, click
4. In the Enter name of new Marker field of the Add Marker dialog box, enter a
name for the new marker, and click
The new marker appears in the Markers field.
If using the SDS Enterprise Database, you cannot create a marker that
Note:
that uses the same combination of marker name and detectors as an existing
marker.
5. Apply detectors to the new marker:
a. In the Markers field, click the new marker to select it.
The software highlights the selected marker.
b. In the Detectors list, select the Use check boxes of the detectors that you
want to assign to the marker.
The software highlights the selected detector.
IMPORTANT! You cannot assign more than two detectors to a marker and the
detectors cannot use the same reporter dye.
6. If evaluating multiple loci, repeat
as needed.
You must configure a marker with two detectors before you can
IMPORTANT!
apply it to a plate document.
Click for information on the features of the Marker Manager dialog
Note:
box or to delete markers from the Markers list.
7. Apply the marker(s) to the allelic discrimination plate document as explained in
"Copying and Applying Markers" on page
DRAFT
.
.
steps 3 through 5 to create additional markers
3-15.
to close it.
Detectors assigned
to 'CYP 2C9*2'