Applied Biosystems 7900HT User Manual page 233

Fast real-time pcr system and sds enterprise database
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Maintaining the Instrument
In This Chapter
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
Notes for Database Users . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-2
Recommended Maintenance Schedule . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-4
Section 7.1 Maintaining the 7900HT Instrument . . . . . . . . . . . . . . . . . . . . . . . . 7-5
Replacing the Sample Block . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-6
Changing the Plate Adapter. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-12
Decontaminating the Sample Block . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-14
Performing a Background Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-16
Performing a Pure Dye Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-20
Adding Custom Dyes to the Pure Dye Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-27
Verifying Instrument Performance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-30
Section 7.2 Maintaining the Plate Handler . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-35
Automation Accessory Components and Stack Positions. . . . . . . . . . . . . . . . . . . 7-36
Adjusting the Sensitivity of the Plate Sensor Switch . . . . . . . . . . . . . . . . . . . . . . 7-37
Aligning the Plate Handler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-41
Aligning the Fixed-Position Bar Code Reader . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-49
Cleaning and Replacing Gripper Finger Pads . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-52
Section 7.3 Maintaining the Computer and Software . . . . . . . . . . . . . . . . . . . 7-53
General Computer Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-54
Maintaining the SDS software. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-55
DRAFT
September 1, 2004 11:39 am, CH_Maintenance.fm
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