Verifying Instrument Performance - Applied Biosystems 7900HT User Manual

Chapter 7 Maintaining the Instrument

Verifying Instrument Performance

When to Perform
Purpose of
RNase P Runs
7-30 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, CH_Maintenance.fm
Applied Biosystems recommends performing an RNase P or TGF-β run:
• When changing sample block formats for the first time
• As needed to verify the function of the 7900HT instrument
The TaqMan RNase P Instrument Verification Plates are experiments run to verify
the performance of the 7900HT instrument for use with optical plates of the same
format (384-Well, 96-Well, or 96-Well Fast). The TaqMan RNase P Instrument
Verification Plates are pre-loaded with the reagents necessary for the detection and
quantification of genomic copies of the human RNase P gene (a single-copy gene
encoding the RNase moiety of the RNase P enzyme). Each well contains pre-loaded
reaction mix (master mix, RNase P primers, and FAM-labeled probe) and template.
Table 7-2 illustrates the arrangement of standards and samples on each type of
TaqMan RNase P Instrument Verification Plate. As shown below, the TaqMan RNase
P Instrument Verification Plates consist of five columns of template standards (1250,
2500, 5000, 10,000, and 20,000 copies) and two unknown populations (5000 and
10,000 copies).
Table 7-2 Configurations of the TaqMan RNase P Instrument Verification Plates
TaqMan RNase P
Instrument Verification
Plate
TaqMan ® RNase P 384-Well
Instrument Verification
Plate (PN 4323306)
DRAFT
Sample Configuration
Unknown 1
5000
Unknown 2
10000