Calibrate The Viia 7 System - Applied Biosystems ViiA 7 Real Quick Reference Manual

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Calibrate the ViiA 7 System
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Calibrate the ViiA
Calibration types
• Regions of interest (ROI)
• Background
• Uniformity
• Dye (only for dyes used in your experiments)
• Normalization
• Instrument verification
Materials required
384-well sample block
• 384-Well Spectral Calibration Plate with FAM
• 384-Well Spectral Calibration Plate with VIC
• 384-Well Spectral Calibration Plate with ROX
• 384-Well Spectral Calibration Plate with SYBR
• 384-Well Spectral Calibration Plate with TAMRA
• 384-Well Spectral Calibration Plate with NED
• 384-Well Region of Interest (ROI) and Background Plates (PN 4432320)
• 384-Well Normalization Plates with FAM
(PN 4432308)
• TaqMan RNase P 384-Well Instrument Verification Plate (PN 4455280)
Array-card sample block
• Array Card Spectral Calibration Dye Kit (PN 4432314)
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• TaqMan RNase P Array Card Instrument Verification Reagents Kit
(PN 4432265)
96-well sample block
• 96-Well Spectral Calibration Plate with FAM
• 96-Well Spectral Calibration Plate with VIC
• 96-Well Spectral Calibration Plate with ROX
• 96-Well Spectral Calibration Plate with SYBR
• 96-Well Spectral Calibration Plate with TAMRA
• 96-Well Spectral Calibration Plate with NED
• 96-Well Region of Interest (ROI) and Background Plates (PN 4432364)
• 96-Well Normalization Plates with FAM
(PN 4432370)
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• TaqMan RNase P 96-Well Instrument Verification Plate (PN 4432382)
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7 System
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Dye (PN 4432271)
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Dye (PN 4432278)
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Dye (PN 4432284)
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Green Dye (PN 4432290)
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Dye (PN 4432296)
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Dye (PN 4432302)
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/ROX and VIC
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Dye (PN 4432327)
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Dye (PN 4432334)
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Dye (PN 4432340)
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Green Dye (PN 4432346)
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Dye (PN 4432352)
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Dye (PN 4432358)
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/ROX and VIC
• ViiA
• Centrifuge with plate adapter
• Powder-free gloves
• Safety goggles
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/ROX Dyes
• ViiA
• Centrifuge with centrifuge array-card carrier
• Powder-free gloves
• Safety goggles
• 200-µL pipettor, with pipette tips
• Array-card staker/sealer fixture
• ViiA
• Centrifuge with plate adapter
• Powder-free gloves
• Safety goggles
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/ROX Dyes
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Applied Biosystems ViiA 7 Real-Time PCR System Quick Reference Guide
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7 System 384-Well Plate Adapter
(PN 4457087)
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7 System Array Card Adapter
(PN 4454166)
clips and buckets
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7 System 96-Well Plate Adapter (PN
4459845)