Sealing The Taqman Low Density Arrays - Applied Biosystems 7900HT User Manual

Sealing the TaqMan Low Density Arrays

Equipment and
Materials Needed
Guidelines for
Sealing the Cards
Positioning the
Sealer
Inserting a Card
Into the Sealer
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
You need the following equipment and materials to seal the Low Density Arrays:
• Microfluidic card sealer
• Sturdy lab bench
• TaqMan Low Density Array(s), containing sample-specific PCR reaction mix
and centrifuged (page
• Scissors
Follow the guidelines below to ensure the Low Density Arrays are properly sealed.
• Seal the Low Density Arrays as soon as possible following centrifugation. The
risk of cross-contamination is minimized by the sealing process.
• Always place the Low Density Arrays into the sealer so that the sealer's carriage
(stylus assembly) travels toward the fill reservoir end of the Low Density Array.
• Always seal in the direction indicated by the arrows etched into the base of the
sealer to ensure that the cDNA sample or control is pushed from the Low
Density Array's channels into its fill reservoirs.
The sealer is best operated by pushing the carriage away from you in a near-to-far
motion (not in a horizontal, side-to-side motion). Follow the procedure below to
correctly position the sealer.
1. Place the sealer on a sturdy lab bench, approximately waist high so that it can be
easily used.
2. Turn the sealer so that the front end (where sealing starts) is closest to you and
the back end is farthest from you.
In the correct position, the arrows on the sealer are pointing away from
Note:
you.
Carriage
1. Place the sealer's carriage in its starting position.
IMPORTANT! Never insert a Low Density Array into the sealer if the carriage is
not in its starting position. If you move the carriage across the Low Density
Array to return it to its starting position, serious damage to the Low Density
Array will occur.
Preparing TaqMan Low Density Arrays for Use
4-16)
Starting
position
DRAFT
September 1, 2004 11:39 am, CH_Basic.fm
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