Applied Biosystems 7900HT User Manual page 173

Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
4. Select Call > Allele X.
The software automatically labels the samples and wells with the Allele X call.
5. Repeat steps 3 through 4
Call Symbol
Allele X
Allele Y
Both
NTC
Undetermined
You can adjust the appearance of the allelic discrimination plot or the
Note:
data points it contains using the Display Settings dialog box. See the Sequence
Detection Systems Software Online Help for more information.
6. If evaluating for multiple markers, do the following:
a. In the Marker drop-down list, select a different marker.
b. Repeat steps 2 through 5
c. Repeat steps a and b until the alleles for each marker have been called.
Calling and Scrutinizing Allelic Discrimination Data
to apply calls to the rest of the samples within the plot.
Definition
●
Homozygous for the allele displayed on the associated
axis of the Allelic Discrimination Plot.
●
●
Heterozygous (Alleles X and Y)
■
No Template Control
✕
Unknown (Unlabeled)
for the new marker.
DRAFT
September 1, 2004 11:39 am, CH_End-Point.fm
Select Allele X
Allele X
homozygotes
Allele Y homozygotes
Allele X/Allele Y
heterozygotes
Allele X homozygotes
No amplification
5-15